Amperometric Glucose Biosensor Based on Pt-Pd Nanoparticles Supported by Reduced Graphene Oxide and Integrated with Glucose Oxidase
Amperometric Glucose Biosensor Based on Pt-Pd Nanoparticles Supported by Reduced Graphene Oxide and Integrated with Glucose Oxidase
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DOI：
10.1002/elan.201400018
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发表时间：
2014-05-01
期刊：
影响因子：
3
通讯作者：
Park, Jae Y.
中科院分区：
文献类型：
--
作者：
Hossain, M. F.;Park, Jae Y.
基于电化学沉积铂钯纳米颗粒(PtPdNPs)固定葡萄糖氧化酶(GOx)，制备了一种新型葡萄糖生物传感器。还原氧化石墨烯(RGO)更多地通过化学和热处理杂化。在RGO表面电化学沉积了双金属纳米颗粒，为PDPT合金在生物传感器制备中的潜在应用奠定了基础。所制备的杂化电极对H_2O_2表现出较高的电催化活性，线性范围为0.5~8 mm(R~2=0.997)，灵敏度为814×10~(-6)A/mm~(-2)(2)。此外，通过一种简单的浇铸方法将葡萄糖氧化酶与活性物质结合在RGO/PdPtNPs表面。该传感器对葡萄糖的线性范围为2~12 mM，灵敏度为24×10~(-6)A/MMCM(2)，检测下限为0.001 mm，响应时间较短(5s)。此外，还考察了干扰物质的影响、传感器的重现性和稳定性。
A novel glucose biosensor was developed based on the immobilization of glucose oxidase (GOx) on reduced graphene oxide incorporated with electrochemically deposited platinum and palladium nanoparticles (PtPdNPs). Reduced graphene oxide (RGO) was more hybridized by chemical and heat treatment. Bimetallic nanoparticles were deposited electrochemically on the RGO surface for potential application of the PdPt alloy in biosensor preparation. The as-prepared hybrid electrode exhibited high electrocatalytic activities toward H2O2, with a wide linear response range from 0.5 to 8mM (R-2=0.997) and high sensitivity of 814x10(-6)A/mMcm(2). Furthermore, glucose oxidase with active material was integrated by a simple casting method on the RGO/PdPtNPs surface. The as-prepared biosensor showed good amperometric response to glucose in the linear range from 2mM to 12mM, with a sensitivity of 24x10(-6)A/mMcm(2), a low detection limit of 0.001mM, and a short response time (5s). Moreover, the effect of interference materials, reproducibility and the stability of the sensor were also investigated.