Investigating the impact of bisphosphonates and structurally related compounds on bacteria containing conjugative plasmids.
Investigating the impact of bisphosphonates and structurally related compounds on bacteria containing conjugative plasmids.
复制标题
研究双膦酸盐和结构相关化合物对含有接合质粒的细菌的影响。
DOI：
10.1016/j.bbrc.2012.07.012
复制
发表时间：
2012
影响因子：
3.1
通讯作者：
Redinbo,MatthewR
中科院分区：
文献类型：
--
作者：
Nash,RebekahP;McNamara,DanE;Ballentine3rd,WKeith;Matson,StevenW;Redinbo,MatthewR
细菌质粒通过接合质粒转移(CPT)的定向过程在微生物种群中繁殖。由于接合质粒通常编码抗生素耐药基因和毒力因子，已描述了几种抑制CPT的方法。双膦酸盐和结构相关化合物(BSRCs)以前被报道破坏F(生育)质粒在大肠杆菌中的接合转移。我们进一步研究了这些化合物对另外两个接合质粒pCU1和R100在大肠杆菌细胞之间转移的影响。研究发现，BSRCs对大肠杆菌存活和转移的影响取决于质粒类型、大肠杆菌暴露在化合物中的时间长短以及质粒供体与受体细胞的比例。因此，这些数据表明，BSRCs对细菌质粒在大肠杆菌中的接合转移产生了一系列的影响。由于它们的影响似乎依赖于质粒类型，BSRCs不太可能作为抗生素耐药性传播的广泛抑制剂。
Bacterial plasmids propagate through microbial populations via the directed process of conjugative plasmid transfer (CPT). Because conjugative plasmids often encode antibiotic resistance genes and virulence factors, several approaches to inhibit CPT have been described. Bisphosphonates and structurally related compounds (BSRCs) were previously reported to disrupt conjugative transfer of the F (fertility) plasmid in Escherichia coli. We have further investigated the effect of these compounds on the transfer of two additional conjugative plasmids, pCU1 and R100, between E. coli cells. The impact of BSRCs on E. coli survival and plasmid transfer was found to be dependent on the plasmid type, the length of time the E. coli were exposed to the compounds, and the ratio of plasmid donor to plasmid recipient cells. Therefore, these data indicate that BSRCs produce a range of effects on the conjugative transfer of bacterial plasmids in E. coli. Since their impact appears to be plasmid type-dependent, BSRCs are unlikely to be applicable as broad inhibitors of antibiotic resistance propagation.