Genetic diversity and discrimination of mango accessions using RAPD and ISSR markers
Genetic diversity and discrimination of mango accessions using RAPD and ISSR markers
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使用 RAPD 和 ISSR 标记鉴定芒果种质的遗传多样性和鉴别
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
R. Chandra
中科院分区：
文献类型：
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作者：
A. Bajpai;N. Srivastava;S. Rajan;R. Chandra
利用RAPD和ISSR标记对46个芒果品种进行了筛选。9个十聚体寡核苷酸和11个简单重复序列分别产生110和160个离散片段。RAPD引物共扩增出14条单态带，96条多态带。这些引物产生的多态性百分比为87.3%。OPA 19、OPA 20和OPC 6是高度多态性的引物。结果表明，该引物扩增出的多态性条带数为14.5条，多态性率为79.38%。ISSR5共扩增出14条多态性带和7条单态性带。UPGMA树构建的RAPD数据的基础上Jaccard的系数分为3类，一个包括大多数的北印度品种和其他的东印度和第三类，包括来自这两个地区的加入。利用UPGMA聚类法不能将供试品种按地理距离进行分类。累积带数据，这两种方法精确地安排加入两个生态地理区域的方式，品种间的亲和力是一致的，我们的理解组。由于东印度和北印度基因型之间的地理距离非常小，因此重叠是合乎逻辑的。两个地区的品种之间没有明确的分离支持共同的基因库起源以及相似的选择压力的操作，因为这些地区的品种偏好基本相同。
Forty six mango cultivars were screened using RAPD and ISSR markers. Nine decamer oligonucleotides and 11 inter simple sequence repeats yielded 110 and 160 discrete fragments, respectively. RAPD primers yielded 14 monomorphic bands and 96 displayed polymorphism. Percent polymorphism generated by these primers was 87.3%. OPA 19, OPA 20 and OPC 6 were highly polymorphic primers. Overall polymorphism detected by ISSR was 79.38%, 14.5 bands per primer. ISSR 5 yielded 14 polymorphic bands and 7 monomorphic bands. UPGMA tree constructed on RAPD data on the basis of Jaccard's coefficient clustered the accessions into 3 groups, one comprising majority of north Indian varieties and other having eastern Indian and third cluster comprising accessions from both the regions. UPGMA clustering of ISSR data could not arrange the cultivars as per geographic separation. Cumulatively band data from these two methods precisely arranged accessions from two ecogeographical regions in such a way that interrcultivar affinities were congruent with our understanding of the group. Since geographic distance between East Indian and North Indian genotypes is very less, it is logical to have overlapping. No clear cut separation among varieties from two regions supports the common gene pool origin as well as operation of similar selection pressure as the cultivar preferences in these areas are largely same.