ROLE OF MOVEMENT IN EMBRYONIC JOINT DEVELOPMENT
ROLE OF MOVEMENT IN EMBRYONIC JOINT DEVELOPMENT
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DOI：
10.1016/0012-1606(66)90022-4
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发表时间：
1966-01-01
影响因子：
2.7
通讯作者：
SOKOLOFF, L
中科院分区：
文献类型：
--
作者：
DRACHMAN, DB;SOKOLOFF, L
为研究肌肉收缩在关节形成中的作用，采用三种方法静脉注射十甲基溴铵麻痹鸡胚；反复静脉注射肉毒杆菌毒素；切除腰骶脊髓的外科手术在7至19天的潜伏期对下肢关节进行组织学研究。关节发育明显异常，3种方法麻痹的胚胎关节发育异常相同。瘫痪肢体的关节腔不能发育，尽管关节区域细胞结构的预备变化正常进行。关节间区首先由纤维维管组织占据，后来由紧密的纤维组织或软骨组织将关节元件结合在一起。关节面变平并扭曲。瘫痪肢体的足底跗骨籽状软骨和关节内韧带未发育。研究表明，实验过程中产生的毒性作用和生长迟缓都不是造成这些异常的原因。在胚胎发育过程中，骨骼肌收缩对初级关节腔的形成、足底跗骨籽状软骨和关节内韧带的形成以及关节表面形状的塑造至关重要。在腔形成阶段之前，肌肉收缩对于关节的形态发生或髌骨籽状软骨的分化都不是必需的。还提出了可能在空腔形成中起辅助作用的其他机制。
To study the role of muscular contractions in the forma-tion of joints, chick embryos were paralyzed by 3 methods intra-venous infusions of decamethonium bromide; repeated intravenous injections of botulinum toxin; surgical extirpation of the lumbosacral spinal cords. The joints of the lower extremities were studied histologically between 7 and 19 days of incubation. Striking abnor-malities of joint development were found, which were identical in the embryos paralyzed by the 3 techniques. Joint cavities failed to develop in the paralyzed limbs, although the preparatory changes in the cel-lular architecture of the joint regions proceeded otherwise normally. The joint interzones were occupied first by fibrovascular tissue, and later by compact fibrous or cartilaginous tissue which bound the artic-ular elements together. The articular surfaces were flattened and distorted. The plantar tarsal sesamoid cartilages and the intraarticular ligaments did not develop in the paralyzed limbs. It has been shown that neither toxic effects nor growth retardation resulting from the experimental procedures were responsible for these abnormalities. Skeletal muscle contractions are essential during embryonic develop-ment for primary joint cavity formation, appearance of the plantar tarsal sesamoid cartilages and intra-articular ligaments, and sculpturing of the form of the articular surfaces. Muscular contractions are not necessary for morphogenesis of articulations prior to the stage of cavity formation, or for differentiation of the patellar sesamoid carti-lage. Additional mechanisms which may play a subsidiary role in cavity formation have been suggested.