The Arabidopsis cohesin protein SYN3 localizes to the nucleolus and is essential for gametogenesis
The Arabidopsis cohesin protein SYN3 localizes to the nucleolus and is essential for gametogenesis
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DOI：
10.1111/j.1365-313x.2007.03106.x
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发表时间：
2007-06-01
期刊：
影响因子：
7.2
通讯作者：
Makaroff, Christopher A.
中科院分区：
文献类型：
--
作者：
Jiang, Ling;Xia, Ming;Makaroff, Christopher A.
α-Kleisin是减数分裂和有丝分裂粘着蛋白复合物的核心组分。拟南芥含有由SYN基因编码的四种α-Kleisin蛋白的基因。SYN 1是REC 8的直系同源物，是减数分裂所必需的，而SYN 2和SYN 4似乎是SCC 1的直系同源物，并在有丝分裂中起作用。我们对AtSYN 3的分析表明，它主要定位于减数分裂和有丝分裂细胞的核仁中。此外，对含有AtSYN 3 T-DNA敲除突变的植物的分析表明，它对巨配子发生是必需的，并在花粉中起着重要作用。这些结果表明，SYN 3可能不是作为典型的粘附素复合物的一部分发挥作用;相反，它可能在控制rDNA结构、转录或rRNA加工中进化出了专门的作用。
alpha-kleisins are core components of meiotic and mitotic cohesin complexes. Arabidopsis contains genes for four alpha-kleisin proteins encoded by SYN genes. SYN1, a REC8 ortholog, is essential for meiosis, while SYN2 and SYN4 appear to be SCC1 orthologs and function in mitosis. Our analysis of AtSYN3 shows that it localizes primarily in the nucleolus of both meiotic and mitotic cells. Furthermore, analysis of plants containing an AtSYN3 T-DNA knockout mutation demonstrated that it is essential for megagametogenesis and plays an important role in pollen. These results suggest that SYN3 may not function as part of a typical cohesin complex; rather it may have evolved a specialized role in controlling rDNA structure, transcription or rRNA processing.