Prevalence of thermophilic Campylobacter infections in humans, chickens and crows in Morogoro, Tanzania
Prevalence of thermophilic Campylobacter infections in humans, chickens and crows in Morogoro, Tanzania
复制标题
DOI：
10.1111/j.1439-0450.2006.00926.x
复制
发表时间：
2006-04-01
期刊：
影响因子：
--
通讯作者：
Kazwala, R. R.
中科院分区：
文献类型：
--
作者：
Mdegela, R. H.;Nonga, H. E.;Kazwala, R. R.
2003年1月至2004年12月期间，在坦桑尼亚莫罗戈罗地区的城市和农村地区进行了一项横断面研究，确定了人类、鸡和乌鸦中嗜热弯曲杆菌感染的流行程度。采用Skirrow方案，对632份人粪便样本、536份本地鸡和肉鸡的肛肠棉签样本以及22份乌鸦的肠道内容物进行了嗜热弯曲杆菌的筛选。采用聚合酶链反应（PCR）方法对人类和鸡标本中具有代表性的空肠弯曲杆菌分离株进行了鉴定。嗜热弯曲杆菌在人类、鸡和乌鸦中的总体患病率分别为9.3% （95% CI: 7.2 ~ 11.9）、69.8% （95% CI: 66.7 ~ 73.6）和72.7% （95% CI: 49.8 ~ 89.3）。在人体内分离到59株嗜热弯曲菌，其中空肠弯曲菌占96.6%，大肠弯曲菌占3.4%。年轻人（16%）的患病率明显高于成年人（7%）（P < 0.001）。341株鸡分离株中，空肠c为91.2%，大肠c为8.8%。农村地方鸡的感染率（76%）显著高于肉鸡（60%）（P < 0.05）。在16株乌鸦中，空肠梭菌占93.8%，大肠梭菌占6.2%。243株空肠梭菌经PCR鉴定阳性，阳性率为74.1%。本研究结果表明，Morogoro的人、鸡和乌鸦中嗜热弯曲杆菌的感染率较高，空肠梭菌的感染率高于大肠杆菌。人类的年龄和鸡的位置被确定为嗜热弯曲杆菌感染的危险因素。生化试验阳性的分离株PCR结果阴性，说明PCR对空肠梭菌的明确诊断有附加价值。
Prevalence of thermophilic Campylobacter infections in humans, chickens and crows was determined in a cross-sectional study that was carried out in urban and rural areas of Morogoro region, Tanzania during the period of January 2003 to December 2004. A total of 632 human stool samples, 536 cloacal swabs from local and broiler chickens and 22 intestinal contents from crows were screened for presence of thermophilic campylobacters using Skirrow's protocol. Representative Campylobacter jejuni isolates from human and chicken samples were also analysed by polymerase chain reaction (PCR) as a definitive identification method. The overall prevalence of thermophilic campylobacters was 9.3% (95% CI: 7.2-11.9), 69.8% (95% CI: 65.7-73.6) and 72.7% (95% CI :49.8-89.3) in humans, chickens and crows respectively. In humans, 59 thermophilic campylobacters were isolated of which 96.6% were C. jejuni and 3.4% Campylobacter coli. There was a significantly (P < 0.001) higher prevalence in young individuals (16%) than in adults (7%). Of 341 isolates from chickens, 91.2% were C. jejuni and 8.8% were C. coli. A significantly (P < 0.05) higher infection rate was observed in rural local chicken (76%) than in broilers (60%). In crows, of 16 isolates, 93.8% were C. jejuni and 6.2% were C. coli. Definitive identification of C. jejuni by PCR revealed positive results in 74.1% of 243 analysed isolates. Findings in this study indicate high prevalence of thermophilic campylobacters in humans, chickens and crows in Morogoro, and a higher infection rate of C. jejuni than that of C. coli in different animal species. Age of humans and location of chickens were identified as risk factors for thermophilic Campylobacter infections. Positive isolates to biochemical tests that indicated negative results on PCR indicates the additional value of PCR for definitive diagnosis of C. jejuni.