Determination of Sulfonamides Antibiotics in Water and Soil Using High Performance Liquid Chromatography
Determination of Sulfonamides Antibiotics in Water and Soil Using High Performance Liquid Chromatography
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高效液相色谱法测定水和土壤中的磺胺类抗生素
DOI：
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发表时间：
2008
影响因子：
1.2
通讯作者：
Yi Ru-Han
中科院分区：
文献类型：
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作者：
Li Yan-Wen;Mo Ce-Hui;Zhao Na;Zhang Rui-Jing;Yi Ru-Han
建立了一种测定土壤和水中磺胺类抗生素的分析方法，水样经HLB柱净化浓缩，土壤样品经甲醇-EDTA-Mcllvaine缓冲液提取，再经LC-SAX和LC-18柱净化浓缩，以乙腈-0.01 mol/L磷酸为移动的流动相，检测波长为270 nm，柱温为25 ℃，流速为10 mL/min，检测波长为270 nm，检测限为10 μ g/mL，检测限为100 μ g/mL。5种磺胺类抗生素在水和土壤中的检出限分别为0.94 ~ 13.2ng/L和0.24 ~ 3.30 μg/kg，土壤样品的加标回收率分别为72.6%~ 85.3%和81.6%~ 105.5%应用该方法测定了污水处理厂水样和蔬菜田土壤样品中的磺胺类抗生素，在珠江三角洲地区，水样中磺胺类药物的总含量为2.26 ~ 12.12 ng/L，土壤中磺胺类药物的总含量为53.68 ~ 155.2 μg/kg。
An analytical method was developed for the determination of sulfonamides antibiotics in soil and water.Water sample was purified and concentrated by HLB cartridge,while soil sample was firstly extracted by a mixture of methanol and EDTA-Mcllvaine buffer,then purified and concentrated by LC-SAX and LC-18 cartridges in tandem.Using acetonitrile and 0.01 mol/L phosphoric acid as the mobile phase,and 270 nm as the detective wavelength,the detective limitation values for 5 sulfonamides antibiotics in water and soil were obtained from 0.94 to 13.2 ng/L and from 0.24 to 3.30 μg/kg respectively.The recoveries were 72.6%-85.3% for soil samples and 81.6%-105.5% for water samples.The method was applied to determine the sulfonamides antibiotics in water samples from sewage treatment plants and soil samples from vegetable fields,Pearl River Delta,South China,that the total amounts of sulfonamides ranged from 2.26 to 12.12 ng/L in water samples and 53.68 to 155.2 μg/kg in soil samples respectively.