Monoclonal ant-idiotypic antibodies reactive with a highly conserved determinant on A/J serum anti-para-azophenylarsonate antibodies.
Monoclonal ant-idiotypic antibodies reactive with a highly conserved determinant on A/J serum anti-para-azophenylarsonate antibodies.
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DOI：
10.1084/jem.154.6.1752
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发表时间：
1981-12-01
影响因子：
15.3
通讯作者：
Nisonoff, A
中科院分区：
文献类型：
--
作者：
Nelles, M J;Gill-Pazaris, L A;Nisonoff, A
先前的报告显示，共享主要交叉反应独特型（CRI）的 A/J 抗对偶氮苯胂酸（抗 Ar）抗体由一系列密切相关但不相同的分子组成。使用 CRI+ 单克隆抗 Ar 抗体进行的血清学研究表明，该家族内存在保守的独特型决定簇。本研究利用了家族内的单克隆抗独特型决定簇。本研究利用单克隆抗独特型抗体来进一步定义保守的独特型决定簇的性质。发现10种CRI+单克隆抗体中的8种具有与三种单克隆抗独特型抗体中的每一种反应的独特型决定簇。此外，大约 60% 的 CRI+ 血清抗 Ar 抗体与一种单克隆抗独特型制剂发生反应。单克隆抗独特型抗体与半抗原结合位点区域的独特位反应，如游离半抗原抑制独特型-抗独特型相互作用的能力所表明的。最后，三种单克隆抗独特型抗体中的两种在抗原之前施用时抑制了随后的 CRE+血清抗 Ar 抗体的产生，而没有显着影响总的抗 Ar 反应。
Previous reports have shown the A/J anti-para-azophenylarsonate (anti- Ar) antibodies that share a major cross-reactive idiotype (CRI) comprise a family of closely related but nonidentical molecules. Serological studies with CRI+ monoclonal anti-Ar antibodies have suggested the presence of a conserved idiotypic determinant within the family. The present study utilized monoclonal ant-idiotypic determinant within the family. The present study utilized monoclonal anti-idiotypic antibodies to define further the nature of the conserved idiotypic determinant. It was found that 8 of 10 CRI+ monoclonal antibodies possess an idiotypic determinant reactive with each of three monoclonal anti-idiotypic antibodies. In addition, approximately 60% of CRI+ serum anti-Ar antibodies reacted with one of the monoclonal anti-idiotypic preparations. The monoclonal anti-idiotypic antibodies react with an idiotope in the region of the hapten-binding site, as indicated by the ability of free haptens to inhibit idiotype-anti-idiotype interactions. Finally, two of three monoclonal anti-idiotypic antibodies suppressed the subsequent production of CRE+ serum anti-Ar antibodies when administered before antigen, without significantly affecting the total anti-Ar response.