Characterization of anti-channel catfish IgL σ monoclonal antibodies
Characterization of anti-channel catfish IgL σ monoclonal antibodies
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DOI：
10.1016/j.vetimm.2010.01.004
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发表时间：
2010-06-15
影响因子：
1.8
通讯作者：
Wilson, Melanie
中科院分区：
文献类型：
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作者：
Edholm, Eva-Stina;Hudgens, Edward D.;Wilson, Melanie
这项研究的特点是三个单克隆抗体（mAb）开发的恒定（C）区的免疫球蛋白轻（IgL）σ链同种型的斑点叉尾鱼，斑点叉尾鱼。利用不同重组（r）蛋白的微球珠测定和蛋白质印迹分析表明，这些抗鲶鱼IgL sigma链mAb各自特异性识别IgL sigma的变性形式。重要的是，使用抗IgL σ mAb的鲶鱼血清的蛋白质印迹法也鉴定了类似于27 kDa的IgL链大小的免疫反应性条带。预期这些mAb与已经存在的抗鲶鱼IG重（H）和IgL链mAb组合将在检查不同鲶鱼IgL同种型的功能作用的未来研究中有用。(C)2010 Elsevier BV保留所有权利。
This study characterizes three monoclonal antibodies (mAbs) developed against the constant (C) region of the immunoglobulin light (IgL) sigma chain isotype of the channel catfish, Ictalurus punctatus. Microsphere bead assays and Western blot analyses utilizing different recombinant (r) proteins show that these anti-catfish IgL sigma chain mAbs each specifically recognize the denatured form of IgL sigma. Importantly, Western blotting of catfish sera using the anti-IgL sigma mAbs also identified an IgL chain-sized immunoreactive band(s) of similar to 27 kDa. It is anticipated that these mAbs in combination with the already existing anti-catfish Ig heavy (H) and IgL chain mAbs will be useful in future studies examining the functional roles of the different catfish IgL isotypes. (C) 2010 Elsevier B.V. All rights reserved.