Effect of tomato extract supplementation against high-fat diet-induced hepatic lesions.
Effect of tomato extract supplementation against high-fat diet-induced hepatic lesions.
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DOI：
10.3978/j.issn.2304-3881.2013.07.04
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发表时间：
2013-08
影响因子：
8
通讯作者：
Wang XD
中科院分区：
文献类型：
--
作者：
Melendez-Martinez AJ;Nascimento AF;Wang Y;Liu C;Mao Y;Wang XD
番茄或番茄制品的摄入量增加与肝癌风险降低有关。在这项研究中，我们研究了补充番茄提取物（TE），其中主要含有番茄红素（LY）和少量的前体，八氢番茄红素（PT）和六氢番茄红素（PTF）对高脂饮食相关的肝脏炎症和脂质分布，以及致癌作用的影响。四组大鼠注射肝脏致癌物二乙基亚硝胺（DEN），然后喂食Lieber-DeCarli对照饮食（35%脂肪，CD）或高脂肪饮食（71%脂肪，HFD），含或不含TE补充剂6周。结果表明，TE的补充显着降低了HFD喂养大鼠肝脏中表达胎盘型谷胱甘肽-S转移酶（p-GST）的炎症灶和肝改变灶（AHF）的多样性。高效液相色谱（HPLC）分析表明，TE补充的结果在一个显着更高的积累PT和PTF比LY在大鼠肝脏。此外，TE补充导致血浆胆固醇水平降低，但肝脏脂质总体增加，这与脂质代谢基因的变化有关，包括过氧化物酶体增殖物激活受体γ（PPARγ）和固醇调节元件结合蛋白（SREBP-1）。这些数据表明，TE补充剂降低了与高膳食脂肪摄入相关的肝脏炎症和血浆总胆固醇。此外，TE补充导致肝脏PT和PTF的积累以及脂肪生成增加，这表明对其生物学功能的进一步研究。
Higher intake of tomatoes or tomato-based products has been associated with lower risk for liver cancer. In this study, we investigated the effects of supplementing tomato extract (TE), which contains mainly lycopene (LY) and less amounts of its precursors, phytoene (PT) and phytofluene (PTF) against high-fat-diet related hepatic inflammation and lipid profiles, and carcinogenesis. Four groups of rats were injected with a hepatic carcinogen, diethylnitrosamine (DEN) and then fed either Lieber-DeCarli control diet (35% fat, CD) or high fat diet (71% fat, HFD) with or without TE supplementation for 6 weeks. Results showed that the supplementation of TE significantly decreased the multiplicity of both inflammatory foci and altered hepatic foci (AHF) expressing placental form glutathione-S transferase (p-GST) in the liver of HFD-fed rats. High-performance liquid chromatography (HPLC) analysis showed that TE supplementation results in a significantly higher accumulation of both PT and PTF than LY in livers of rats. In addition, the TE supplementation led to a decrease of plasma cholesterol levels but an overall increase in hepatic lipids which is associated with changes in the genes on lipid metabolism, including the peroxisome proliferator-activated receptor gamma (PPARγ) and the sterol-regulatory element binding protein (SREBP-1). These data suggest that TE supplementation decreases hepatic inflammation and plasma total cholesterol associated with high dietary fat intake. Moreover, TE supplementation results in an accumulation of hepatic PT and PTF as well as increased lipogenesis suggesting further investigation into their biological function(s).