A comparison of the community diversity of foliar fungal endophytes between seedling and adult loblolly pines (Pinus taeda).
A comparison of the community diversity of foliar fungal endophytes between seedling and adult loblolly pines (Pinus taeda).
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DOI：
10.1016/j.funbio.2015.07.003
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发表时间：
2015-10
期刊：
影响因子：
2.5
通讯作者：
Lutzoni F
中科院分区：
文献类型：
--
作者：
Oono R;Lefèvre E;Simha A;Lutzoni F
真菌内生菌是地球上最普遍存在的植物共生体之一，在系统发育上具有多样性。植物内生菌群落的结构和多样性与寄主分类群和气候有关，但对寄主成熟期内生菌群落的研究相对较少。采用培养法和非培养法比较了不同季节、不同地点火炬松（Pinus taeda）幼苗和成树叶片真菌内生菌群落。我们对内部转录间隔区和邻近的部分大亚基核糖体RNA基因（ITS-LSU扩增子）进行测序，以划定操作分类单位并对群落进行系统发育表征。尽管与成树相比，幼苗的侵染频率较低，但幼苗针叶能接受更多样化的真菌内生菌群落。无培养法证实了成虫针叶中常见的otu，但在幼苗针叶中发现了几种培养法未发现的新otu。在成虫体内最常见的两种OTUs很少从幼苗中培养出来，这表明寄主年龄与特定内生菌的选择性富集有关。这种内生真菌物种优势的转变可能表明这些真菌和它们的火炬松宿主之间的功能变化。
Fungal endophytes represent one of the most ubiquitous plant symbionts on Earth and are phylogenetically diverse. The structure and diversity of endophyte communities have been shown to depend on host taxa and climate, but there have been relatively few studies exploring endophyte communities throughout host maturity. We compared foliar fungal endophyte communities between seedlings and adult trees of loblolly pines (Pinus taeda) at the same seasons and locations by culturing and culture-independent methods. We sequenced the internal transcribed spacer region and adjacent partial large subunit nuclear ribosomal RNA gene (ITS–LSU amplicon) to delimit operational taxonomic units and phylogenetically characterize the communities. Despite the lower infection frequency in seedlings compared to adult trees, seedling needles were receptive to a more diverse community of fungal endophytes. Culture-free method confirmed the presence of commonly cultured OTUs from adult needles but revealed several new OTUs from seedling needles that were not found with culturing methods. The two most commonly cultured OTUs in adults were rarely cultured from seedlings, suggesting that host age is correlated with a selective enrichment for specific endophytes. This shift in endophyte species dominance may be indicative of a functional change between these fungi and their loblolly pine hosts.