Periodontal regeneration of transplanted rat molars after cryopreservation
Periodontal regeneration of transplanted rat molars after cryopreservation
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DOI：
10.1016/j.archoralbio.2003.07.002
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发表时间：
2004-01-01
影响因子：
3
通讯作者：
Ozawa, H
中科院分区：
文献类型：
--
作者：
Kawasaki, N;Hamamoto, Y;Ozawa, H
深低温保存对牙周组织的影响。对大鼠磨牙移植后的再生进行了组织学和组织化学观察。取4周龄Wistar大鼠双侧上颌第一、第二磨牙，轻轻拔除，立即或液氮冷冻过夜后移植于腹部皮下结缔组织。以5%二甲基亚砜（DMSO）和6%羟乙基淀粉（HES）为冷冻保护剂，在程序冷冻机中缓慢冷冻供体牙齿。移植后1 - 4周，将它们与周围组织一起小心地切除。牙周无细胞牙骨质再生。即刻移植后2周观察骨组织、韧带、牙槽骨的变化。牙髓的修复是由根尖肉芽组织向内生长，随后形成钙化组织。再生牙周。韧带碱性磷酸酶（ALP）阳性。4周时，在新生牙槽骨和牙髓硬组织上散在分布着小的或单核的抗酒石酸酸性磷酸酶（TRAP）阳性细胞，但无外部或内部进行性牙根吸收。冷冻保存后1周牙骨质表面粗糙，但随着成牙骨质细胞的增多，牙周膜和牙槽骨的出现，3周牙骨质表面变得光滑。Malassez的上皮剩余：（ERM）也恢复。牙周组织再生后4周，没有证据表明牙根吸收。虽然这个过程进展缓慢，冷冻保存的牙齿显示牙周。再生基本上类似于立即移植的牙齿，没有进行性牙根吸收，表明它们可以适用于临床使用。(C)2003 Elsevier Ltd.保留所有权利。
The effects of cryopreservation on periodontal. regeneration of transplanted rat molars were investigated histologically and histochemically in rats. Bilateral first and second maxillary molars of 4-week-old Wistar rats were gently extracted and transplanted into the abdominal subcutaneous connective tissue immediately or after cryopreservation in liquid nitrogen overnight. Donor teeth were slowly frozen by a rate-controlling freezer (program freezer) using 5% dimethylsulfoxide (DMSO) and 6% hydroxyethyl starch (HES) as cryoprotectants. One-four weeks after transplantation, they were carefully excised with the surrounding tissues. Regeneration of acellular cementum, periodontal. ligament, and alveolar bone were observed 2 weeks after immediate transplantation. The pulp was repaired by the ingrowth of granulation tissue from the root apex followed by the formation of calcified tissue. The regenerated periodontal. ligament was positive for alkaline phosphatase (ALP). Small or mononuclear tartrate resistant acid phosphatase (TRAP) positive cells were scattered on the newly formed alveolar bone and on the hard tissue in the pulp, but there was no external or internal progressive root resorption at 4 weeks. Cryopreserved teeth had acellular cementum with a rough surface at 1 week, but with the increase of cementoblasts and the appearance of periodontal ligament and alveolar bone, the surface became smooth at 3 weeks. Epithelial rests of Malassez: (ERM) also revived. After regeneration of the periodontal tissues at 4 weeks, there was no evidence of root resorption. Although the process proceeded slowly, the cryopreserved teeth showed the periodontal. regeneration substantially similar to that of the immediately transplanted teeth without progressive root resorption, indicating that they could be applicable for clinical use. (C) 2003 Elsevier Ltd. All rights reserved.