Aging and lymphokine gene expression by T cell subsets.
Aging and lymphokine gene expression by T cell subsets.
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T 细胞亚群的衰老和淋巴因子基因表达。
DOI：
10.1111/j.1753-4887.1995.tb01511.x
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发表时间：
2009
影响因子：
6.1
通讯作者：
M. Hobbs
中科院分区：
文献类型：
--
作者：
D. Ernst;O. Weigle;M. Hobbs
我将描述的工作是在斯克里普斯诊所和研究基金会免疫学系进行的。在我们的实验室中，我们感兴趣的是确定衰老如何影响 T 细胞依赖性免疫的细胞和分子机制。我们使用了幼稚/记忆 T 细胞范例（图 1）。初次接触抗原后，初始 T 细胞表达早期反应基因，包括编码 T 细胞生长因子、白细胞介素 2 (IL-2) 和 IL-2 受体链的基因。 IL-2 通过高亲和力 IL-2 受体驱动活化细胞增殖并分化为效应 T 细胞，介导初级体液或细胞介导的免疫反应。在此过程中，会产生大量分化的抗原特异性 T 细胞。它们被识别为记忆细胞。由于其分化状态，记忆 T 细胞比初始 T 细胞表达更高水平和更多种类的淋巴因子（也称为细胞因子）；前者比初始 T 细胞更有效地产生和调节体液和细胞介导的免疫反应以回忆抗原。除了细胞因子库的变化之外，幼稚细胞向记忆细胞的转变还伴随着各种膜分子表达水平的量和质的变化，这可能导致细胞功能的改变。其中一些分子可用于鉴定和分离小鼠系统中的初始 T 细胞和记忆 T 细胞，包括 CD44、CD45R 和 CD62L（小鼠 MEL-14）（表 I）。初始 T 细胞以低至中等水平表达 CD44 粘附分子；它的表达在抗原刺激后上调，并且此后似乎由记忆 T 细胞稳定表达。针对 CD45 不同亚型的单克隆抗体，其表达仅限于特定有核细胞 (CD45R)
The work I will describe was conducted at the Department of Immunology, Scripps Clinic and Research Foundation. In our laboratory we are interested in determining how aging affects the cellular and molecular mechanisms that underlie T cell-dependent immunity. We have used the Naive/Memory T Cell Paradigm (Figure 1). Following a primary encounter with antigen, naive T cells express early response genes, including those encoding the T cell growth factor, interleukin-2 (IL-2), and the IL-2 receptor chains. IL-2 works through high affinity IL-2 receptors to drive activated cells to proliferate and differentiate into effector T cells that mediate primary humoral or cell-mediated immune responses. In the process, a larger number of differentiated, antigen-specific T cells are generated. They are identified as memory cells. As a consequence of their differentiated state, memory T cells can express higher levels and a greater variety of lymphokines (also known as cytokines) than naive T cells; the former more efficiently generate and regulate humoral and cell-mediated immune responses to recall antigen than naive T cells. In addition to changes in cytokine repertoires, the naive-to-memory cell conversion is accompanied by quantitative and qualitative changes in the expressed levels of various membrane molecules that can contribute to altered cell function. Several of these molecules have been useful in identifying and isolating naive and memory T cells in the mouse system, including CD44, CD45R, and CD62L (mouse MEL-14)(Table I). The CD44 adhesion molecule is expressed at low to intermediate levels by naive T cells; its expression is upregulated upon antigenic stimulation and appears to be stably expressed thereafter by memory T cells. Monoclonal antibodies directed against different isoforms of CD45 whose expression is restricted (CD45R) to particular nucleated
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影响因子：
4.4
作者：
M. Hobbs;W. Weigle;D. Noonan;B. Torbett;R. Mcevilly;Rick Koch;G. Cardenas;D. Ernst
通讯作者：
M. Hobbs;W. Weigle;D. Noonan;B. Torbett;R. Mcevilly;Rick Koch;G. Cardenas;D. Ernst
DOI：
10.1016/0167-5699(89)90060-1
发表时间：
1989
期刊：
Immunology today
影响因子：
--
作者：
J. Sprent;M. Schaefer
通讯作者：
J. Sprent;M. Schaefer
小鼠 CD8 T 细胞 IFN-γ 合成的年龄相关增加与膜 CD44、CD45RB、3G11 和 MEL-14 表达定义的细胞亚群频率的变化相关。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ernst,DN;Weigle,WO;Noonan,DJ;McQuitty,DN;Hobbs,MV
通讯作者：
Hobbs,MV
通过转化生长因子-β 1 差异激活 CD8 T 细胞。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lee,HM;Rich,S
通讯作者：
Rich,S