Low dietary soy isoflavonoids increase hippocampal spine synapse density in ovariectomized rats.
Low dietary soy isoflavonoids increase hippocampal spine synapse density in ovariectomized rats.
复制标题
低饮食大豆异黄酮会增加卵巢切除大鼠的海马棘突触密度。
DOI：
10.1016/j.brainres.2017.01.002
复制
发表时间：
2017-02-15
期刊：
影响因子：
2.9
通讯作者：
Leranth C
中科院分区：
文献类型：
--
作者：
MacLusky NJ;Thomas G;Leranth C
饮食中大量摄入植物雌激素(植物雌激素)会影响大脑的结构和功能。然而，在正常的动物和人类饮食摄入量范围内摄入植物雌激素的影响仍然不确定。本研究的目的是确定标准低植物雌激素实验大鼠饲料中存在的异黄酮类化合物对海马区CA1区放射层中脊髓突触密度的影响。断奶大鼠(22日龄)被喂养40天，要么是标准饲料(Tekraad 2018)，要么是不含大豆的营养相似的饲料(Tekraad 2016)，要么是含有主要大豆异黄酮类化合物大豆苷元和染料木素的定制饲料。54日龄大鼠摘除卵巢。8天后，用电子显微镜体视学分析方法测量CA1区放射层内海马锥体神经元顶端树突上的突触密度。在Tekraad 2016上维护的动物表现出比使用Tekraad 2018的动物CA1脊椎突触密度低约60%。在特克拉德2016年中更换染料木素和大豆苷元，使突触密度恢复到与特克拉德2018年中动物的突触密度几乎无法区分的水平。这些结果表明，标准实验大鼠饲料中的异黄酮类化合物对海马区CA1区的脊髓突触密度有显著影响。由于海马区这一区域脊椎突触密度的变化与认知表现和情绪状态有关，这些数据表明，即使每天摄入相对较少的大豆植物雌激素，也可能足以影响海马体功能。
High dietary intake of plant estrogens (phytoestrogens) can affect brain structure and function. The effects of phytoestrogen intake within the range of normal animal and human dietary consumption, however, remain uncertain. The aim of the present study was to determine the effects of the isoflavonoids present in a standard low phytoestrogen laboratory rat chow on spine synapse density in the stratum radiatum of area CA1 of the hippocampus. Weanling rats (22 days old) were fed either standard chow (Teklad 2018), a nutritionally comparable diet without soy (Teklad 2016) or a custom diet containing Teklad 2016 supplemented with the principal soy isoflavonoids, daidzein and genistein, for 40 days. Rats were ovariectomized at 54 days of age. Eight days later, spine synapse density on the apical dendrites of hippocampal pyramidal neurons in the stratum radiatum of area CA1 was measured by electron microscopic stereological analysis. Animals maintained on Teklad 2016 exhibited an approximately 60% lower CA1 spine synapse density than animals consuming Teklad 2018. Replacing genistein and daidzein in Teklad 2016 returned synapse density to levels indistinguishable from those in animals on Teklad 2018. These results indicate that the isoflavonoids in a standard laboratory rat diet exert significant effects on spine synapse density in the CA1 region of the hippocampus. Since changes in spine synapse density in this region of the hippocampus have been linked to cognitive performance and mood state, these data suggest that even relatively low daily consumption of soy phytoestrogens may be sufficient to influence hippocampal function.