Biospecific Recognition of Tethered Small Molecules Diluted in Self‐Assembled Monolayers
Biospecific Recognition of Tethered Small Molecules Diluted in Self‐Assembled Monolayers
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自组装单层稀释的束缚小分子的生物特异性识别
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
A. M. Andrews
中科院分区：
文献类型：
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作者：
M. Shuster;A. Vaish;Matthew E. Szapacs;M. E. Anderson;P. S. Weiss;A. M. Andrews
利用插入自组装和化学功能化的结合，开发了一种通过小分子探针捕获大生物分子靶标的模型系统。小分子的选择性分子识别的关键是能够束缚这些小分子，使得它们易于结合。这是特别具有挑战性的，因为系链改变了探针分子的显著部分。间隔小分子探针也是至关重要的，使得它们被稀释和暴露，而不是通过捕获表面上的相分离聚集，特别是当试图结合大分子靶标（例如，蛋白质、核酸）。[1-5]最佳稀释避免了空间位阻[6-8]和多价非特异性相互作用的影响。稀释的表面覆盖对于实现防止非特异性结合的策略也很重要。我们已经开发了一种解决这些问题的通用方法[5，9 -12]，并将其应用于用小分子神经递质血清素（5-羟色胺;图1）功能化的表面。5-羟色胺与以稀释覆盖率插入到预先存在的具有寡聚（乙二醇）分子表面的自组装单分子层（SAM）中的系链共价结合。我们选择5-羟色胺作为最初的探针，因为它是一种重要的神经递质，与焦虑和情绪有关，
A model system for the capture of large biomolecule targets by small-molecule probes has been developed using a combination of insertion self-assembly and chemical functionalization. Key to selective molecular recognition of small molecules is the ability to tether these such that they are readily accessible for binding. This is particularly challenging because the tether alters a significant fraction of the probe molecule. It is also critical to space small-molecule probes so that they are dilute and exposed, rather than clustered via phase separation on capture surfaces, particularly when trying to bind large-molecule targets (e.g., proteins, nucleic acids). [1–5] Optimal dilution avoids the effects of steric hindrance [6–8] and multivalent non-specific interactions. Dilute surface coverage is also important to enable strategies to prevent nonspecific binding. We have developed a general method for addressing these problems [5,9–12] and apply these to surfaces functionalized with the small-molecule neurotransmitter serotonin (5-hydroxytryptamine; Fig. 1). Serotonin is covalently bound to tethers inserted at dilute coverage into pre-existing self-assembled monolayers (SAMs) bearing a molecular surface of oligo(ethylene glycol). We chose serotonin as our initial probe both because of its important roles as a neurotransmitter involved in anxiety and mood
影响因子：
7.4
作者：
Wegner, GJ;Lee, HJ;Corn, RM
通讯作者：
Corn, RM
影响因子：
7.4
作者：
Lahiri, J;Isaacs, L;Whitesides, GM
通讯作者：
Whitesides, GM