To hum or not to hum: Neural transcriptome signature of male courtship vocalization in a teleost fish
To hum or not to hum: Neural transcriptome signature of male courtship vocalization in a teleost fish
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DOI：
10.1111/gbb.12740
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发表时间：
2021-06-09
影响因子：
2.5
通讯作者：
Bass，Andrew H.
中科院分区：
文献类型：
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作者：
Tripp，Joel A.;Feng，Ni Y.;Bass，Andrew H.
对于许多动物物种来说，发声交流是一种关键的社会行为，通常也是繁殖成功的必要组成部分。此外，发声通常是对运动动作要求很高。为了知道发声是否需要特定的分子工具包，我们使用RNA测序来研究一种极端发声行为的神经基因表达，这种极端发声行为是平原学员鱼(Porichthys Notatus)的求偶嗡嗡声。单次哼唱可以持续长达2 h，并可能在整个晚上的求爱活动中重复。我们通过比较哼唱和非哼唱男性的转录表达水平，询问发声行为状态是否与大脑关键区域中调节发声的特定基因表达特征有关。我们发现，在哼唱时，与昼夜节律相关的基因周期3和时钟分别在发声运动核团和视前区-下丘脑前部显著上调，这表明在这些不同的行为状态下，体内的生物钟可能有所不同。此外，我们还鉴定了一组差异表达的基因，这些基因与突触传递、离子通道和运输、神经肽和激素信号以及代谢和抗氧化活性有关，这些基因可能共同支持哼唱行为的神经和能量需求。不同区域转录表达的比较强调了大脑基因表达的区域差异，同时也表明，在为求爱行为做准备的声带运动回路中，基因调节是协调的。这些结果强调了差异基因表达在行为状态转换中的作用，在这种情况下，神经内分泌、运动和昼夜节律控制求偶发声。
For many animal species, vocal communication is a critical social behavior and often a necessary component of reproductive success. Additionally, vocalizations are often demanding motor acts. Wanting to know whether a specific molecular toolkit might be required for vocalization, we used RNA‐sequencing to investigate neural gene expression underlying the performance of an extreme vocal behavior, the courtship hum of the plainfin midshipman fish (Porichthys notatus). Single hums can last up to 2 h and may be repeated throughout an evening of courtship activity. We asked whether vocal behavioral states are associated with specific gene expression signatures in key brain regions that regulate vocalization by comparing transcript expression levels in humming versus non‐humming males. We find that the circadian‐related genesperiod3andClockare significantly upregulated in the vocal motor nucleus and preoptic area‐anterior hypothalamus, respectively, in humming compared with non‐humming males, indicating that internal circadian clocks may differ between these divergent behavioral states. In addition, we identify suites of differentially expressed genes related to synaptic transmission, ion channels and transport, neuropeptide and hormone signaling, and metabolism and antioxidant activity that together may support the neural and energetic demands of humming behavior. Comparisons of transcript expression across regions stress regional differences in brain gene expression, while also showing coordinated gene regulation in the vocal motor circuit in preparation for courtship behavior. These results underscore the role of differential gene expression in shifts between behavioral states, in this case neuroendocrine, motor and circadian control of courtship vocalization.