A Microfluidic Culturing System for Observation of Free-Floating Microorganisms
A Microfluidic Culturing System for Observation of Free-Floating Microorganisms
复制标题
用于观察自由漂浮微生物的微流体培养系统
DOI：
10.1115/icnmm2013-73164
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Y. Matsumoto
中科院分区：
文献类型：
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作者：
K. Mogi;Y. Sugii;T. Fujii;Y. Matsumoto
在微通道环境下，观察了乙醇对布劳尼芽孢杆菌个体细胞的毒性作用。为了在微通道环境中连续观察布氏杆菌，研制了一种具有特定通道结构的微流控装置。利用通道结构，微生物按大小进行分类，并在通道中相应的区域进行捕获。在实验中，将含有DAPI的布朗氏杆菌悬液注射到装置中，用于实验中的细胞捕获。细胞捕获后，将含0.5% DAPI的乙醇注入通道内60分钟。损伤细胞通过变形和DAPI荧光检测证实。20分钟后证实DAPI有强荧光。另一方面，与DAPI的荧光相比，叶绿体的荧光变弱。我们成功地确定了微通道中乙醇对布朗杆菌暴露的时间条件。ASME版权所有©2013
We observed the effect of the ethanol toxicity on individual cells of Botryococcus braunii (B. braunii) in microchannel environment. For continuous observation of B. braunii in microchannel environment, a microfluidic device which has a specific channel structure was developed. By using the channel structure, microorganisms were sorted by their size and captured on the corresponding area in the channel. In the experiment, suspension of B. braunii with DAPI was injected into the device for the cell capturing in the experiment. Following the cell capturing, ethanol with DAPI 0.5% was injected into the channel for 60 minutes. Damaged cells were confirmed by their deformation and the fluorescence of DAPI. The strong fluorescence of DAPI was confirmed after 20 minutes. On the other hand, the fluorescence of the chloroplast became weak in contrast to the fluorescence of DAPI. We successfully confirmed the time condition of exposing B. braunii to ethanol in the micro channel.Copyright © 2013 by ASME