Biodegradable Polyphosphazene-Nanohydroxyapatite Composite Nanofibers: Scaffolds for Bone Tissue Engineering
Biodegradable Polyphosphazene-Nanohydroxyapatite Composite Nanofibers: Scaffolds for Bone Tissue Engineering
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DOI：
10.1166/jbn.2009.032
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发表时间：
2009-02-01
影响因子：
2.9
通讯作者：
Laurencin, Cato T.
中科院分区：
文献类型：
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作者：
Bhattacharyya, Subhabrata;Kumbar, Sangamesh G.;Laurencin, Cato T.
骨是一种天然的复合材料，由纳米级的胶原纤维、羟基磷灰石和蛋白多糖组成。本研究采用静电纺丝技术制备了可生物降解的聚双（乙基丙氨酰）磷腈-纳米羟基磷灰石（PNEA-nHAp）复合材料。使用THF和乙醇的二元溶剂组合物作为纺丝溶剂以在PNEA溶液中获得更好的纳米羟基磷灰石结晶。这些纳米复合材料的形态，nHAp的分布和内容，使用光谱学和重量分析法进行了表征。在100-310 nm的直径范围内制备的复合纳米纤维可以包裹20-40 nm的nHAp晶体。对于50%（w/w）nHAp实验负载量，获得了更好的复合物热稳定性产率。增量实验负载超过60%（W/W）阻碍静电纺丝由于聚合物-nHAp相分离。复合材料纳米纤维具有较粗糙的表面和结节沿着纤维的长度，表明nHAp封装。此外，通过能量色散X射线光谱和X射线成像的表征证实了nHAp包封。为细胞提供具有纤维状结构和天然羟基磷灰石的天然骨样环境可以增强骨组织再生/修复。
Bone is a natural composite comprised of hierarchically arranged collagen fibrils, hydroxyapatite and proteoglycans in the nanometer scale. This preliminary study reports the fabrication of biodegradable poly[bis(ethyl alanato)phosphazene]-nanohydroxyapatite (PNEA-nHAp) composite nanofiber matrices via electrospinning. Binary solvent compositions of THF and ethanol were used as a spinning solvent to attain better nanohydroxyapatite dispersibility in PNEA solution. These nanocomposites were characterized for morphology, nHAp distribution and content using spectroscopy and gravimetric estimations. Composite nanofibers fabricated in the diameter range of 100-310 nm could encapsulate 20-40 nm nHAp crystals. A better composite nanofiber yield was obtained for 50% (w/w) nHAp experimental loadings. Incremental experimental loading beyond 60% (W/W) hindered electrospinning due to polymer-nHAp phase separation. Composites nanofibers had a rougher surface and nodules along the length of the fibers suggesting nHAp encapsulation. Further, characterization via energy dispersive X-ray spectroscopy and X-ray mapping confirmed the nHAp encapsulation. Providing cells with a natural bone like environment with a fibrillar structure and natural hydroxyapatite can enhance bone tissue regeneration/repair.