Week-long imaging of cell divisions in the Arabidopsis root meristem
Week-long imaging of cell divisions in the Arabidopsis root meristem
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DOI：
10.1186/s13007-019-0417-9
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发表时间：
2019-03-25
期刊：
影响因子：
5.1
通讯作者：
Birnbaum, Kenneth D.
中科院分区：
文献类型：
--
作者：
Rahni, Ramin;Birnbaum, Kenneth D.
描述动态系统的行为需要以高的时间和空间分辨率捕获它们。由于其透明性和遗传易处理性，拟南芥根适合与细胞和组织特异性荧光报告分子结合时进行活体成像。我们开发了一种新的4D成像方法，利用简单的共聚焦显微镜和容易获得的组件来跟踪细胞分裂在根干细胞龛和周围区域长达1 week.使用这种方法，我们进行了直接测量细胞分裂间隔内和周围的根干细胞龛。结果显示，在近端干细胞的细胞分裂率的一个短，陡峭的梯度，从静止中心（QC），细胞直接接触的QC（初始），他们的直接女儿，分裂率似乎变得更加homogeneous.These结果提供了一个基线，以研究如何扰动信号可能会影响细胞分裂模式在根分生组织的细胞分裂速率逐渐更快。这种新的设置进一步使我们能够精细地分析导致图案化的分生组织细胞分裂速率。
Characterizing the behaviors of dynamic systems requires capturing them with high temporal and spatial resolution. Owing to its transparency and genetic tractability, the Arabidopsis thaliana root lends itself well to live imaging when combined with cell and tissue-specific fluorescent reporters. We developed a novel 4D imaging method that utilizes simple confocal microscopy and readily available components to track cell divisions in the root stem cell niche and surrounding region for up to 1 week.Using this method, we performed a direct measurement of cell division intervals within and around the root stem cell niche. The results reveal a short, steep gradient of cell division rates in proximal stem cells, with progressively more rapid cell division rates from quiescent center (QC), to cells in direct contact with the QC (initials), to their immediate daughters, after which division rates appear to become more homogeneous.These results provide a baseline to study how perturbations in signaling could affect cell division patterns in the root meristem. This new setup further allows us to finely analyze meristematic cell division rates that lead to patterning.