Low and high-dose intradermal infection with Leishmania majorand Leishmania amazonensis in C57BL/6 mice
Low and high-dose intradermal infection with Leishmania majorand Leishmania amazonensis in C57BL/6 mice
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DOI：
10.1590/s0074-02762010000600002
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发表时间：
2010-09-01
期刊：
影响因子：
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通讯作者：
Vieira, Leda Quercia
中科院分区：
文献类型：
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作者：
Côrtes, Denise Fonseca;Carneiro, Matheus Batista Heitor;Vieira, Leda Quercia
用低剂量的寄生虫感染亚马逊利什曼原虫的皮肤模型与用高剂量的利什曼原虫感染的模型进行比较。亚马逊线虫和低剂量和高剂量的硕大利什曼原虫。用103或10（6）个寄生虫感染C57 BL/6小鼠的耳部，并评估感染的结果。与感染10（6）利什曼原虫的小鼠相比，感染10（3）寄生虫的小鼠中病变的出现延迟，并且在病变变得明显之前在感染部位可检测到寄生虫。感染L. amazonensis表现为持续性病变，而L.在所有组中，主要的自发愈合，尽管淋巴细胞在愈合后仍然存在于感染部位。仅在L.感染亚马逊河病毒的小鼠高剂量L.感染亚马逊河原虫的小鼠产生的IFN-γ和TNF水平低于感染亚马逊河原虫的小鼠。少校寄生虫的持久性与IL-10水平和巨噬细胞产生一氧化氮或尿素之间没有相关性。真皮中的亚马逊病毒通过延迟损伤的出现而改变感染的过程。然而，低剂量感染不会改变皮下感染大量寄生虫的易感性和细胞因子产生的结果。
A model of skin infection with Leishmania amazonensis with low doses of parasites is compared to infection with high doses of L. amazonensis and low and high doses of Leishmania major. C57BL/6 mice were infected with 103 or 10(6) parasites in the ear and the outcome of infection was assessed. The appearance of lesions in mice infected with 10(3) parasites was delayed compared to mice infected with 10(6) Leishmania and parasites were detectable at the infection site before lesions became apparent. Mice infected with L. amazonensis displayed persistent lesions, whereas infection with L. major spontaneously healed in all groups, although lymphocytes persisted at the site of infection after healing. Macrophages persisted only in L. amazonensis-infected mice. High-dose L. amazonensis-infected mice produced lower levels of IFN-gamma and TNF than mice infected with L. major. No correlation between the persistence of parasites and IL-10 levels and the production of nitric oxide or urea by macrophages was found. We conclude that infection with low doses of L. amazonensis in the dermis changes the course of infection by delaying the appearance of lesions. However, low-dose infection does not change the outcomes of susceptibility and cytokine production described for subcutaneous infection with high numbers of parasites.