Evaluation of bactericidal effects of low‐temperature nitrogen gas plasma towards application to short‐time sterilization
Evaluation of bactericidal effects of low‐temperature nitrogen gas plasma towards application to short‐time sterilization
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DOI：
10.1111/j.1348-0421.2012.00457.x
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发表时间：
2012-07
影响因子：
2.6
通讯作者：
K. Kawamura;Ayaka Sakuma;Yuka Nakamura;Tomoko Oguri;Natsumi Sato;N. Kido
中科院分区：
文献类型：
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作者：
K. Kawamura;Ayaka Sakuma;Yuka Nakamura;Tomoko Oguri;Natsumi Sato;N. Kido
为了开发一种使用纯氮气作为等离子体源的新型低温等离子体灭菌器，我们对NGK绝缘体公司提供的原型装置的杀菌能力进行了评估。测定无冷点灭菌条件后，在滤纸上测定嗜热硬脂嗜热地杆菌内生孢子的BI D值为1.9 min。然而，携带相同内生孢子的BI在SUS上的灭活效率存在一定的差异，这表明灭菌器具的材料不同，灭菌效果也不同。金黄色葡萄球菌和大肠杆菌也暴露在氮气等离子体中，并在30分钟内被证实灭活。通过对灭菌袋杀菌效率的评价，得出等离子体中的紫外光子和产生气体等离子体的高压脉冲与氮气气体等离子体的杀菌效果无关。活性氮原子或分子自由基可能具有杀菌作用。
To develop a novel low‐temperature plasma sterilizer using pure N2 gas as a plasma source, we evaluated bactericidal ability of a prototype apparatus provided by NGK Insulators. After determination of the sterilizing conditions without the cold spots, the D value of the BI of Geobacillus stearothermophilus endospores on the filter paper was determined as 1.9 min. However, the inactivation efficiency of BI carrying the same endospores on SUS varied to some extent, suggesting that the bactericidal effect might vary by materials of sterilized instruments. Staphylococcus aureus and Escherichia coli were also exposed to the N2 gas plasma and confirmed to be inactivated within 30 min. Through the evaluation of bactericidal efficiency in a sterilization bag, we concluded that the UV photons in the plasma and the high‐voltage pulse to generate the gas plasma were not concerned with the bactericidal effect of the N2 gas plasma. Bactericidal effect might be exhibited by activated nitrogen atoms or molecular radicals.