Engineering ketocarotenoid biosynthesis in potato tubers
Engineering ketocarotenoid biosynthesis in potato tubers
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DOI：
10.1016/j.ymben.2006.01.001
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发表时间：
2006-05-01
影响因子：
8.4
通讯作者：
Taylor, Mark A.
中科院分区：
文献类型：
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作者：
Morris, Wayne L.;Ducreux, Laurence J. M.;Taylor, Mark A.
消费虾青素越来越多地与一系列健康益处相关。尽管没有关于植物贮藏器官中营养显著水平的虾青素的报道，但在植物中工程化酮类胡萝卜素生物合成的尝试已经成功。因此，在这项研究中，酮类胡萝卜素的生物合成工程马铃薯块茎。马铃薯和茄phureja转基因株系，表达藻类bkt 1基因，编码β-酮醇酶，并积累酮类胡萝卜素。检测到两种主要的酮类胡萝卜素，酮黄素和虾青素。未酯化的虾青素含量达到约。14 μ g g g（-1）DW。而在S.块茎背景。共转化S.用crtB（编码八氢番茄红素合成酶）和bkt 1基因对马铃薯进行了重组，以确定这是否会提高S.块茎酮类胡萝卜素(C)2006年爱思唯尔公司All rights reserved.
Consumption of astaxanthin is increasingly associated with a range of health benefits. Attempts to engineer ketocarotenoid biosynthesis in plants have been successful although there are no reports of nutritionally significant levels of astaxanthin in plant storage organs. Thus, in this study, ketocarotenoid biosynthesis was engineered in potato tubers. Both Solanum tuberosum and Solanum phureja transgenic lines were produced that expressed an algal bkt1 gene, encoding a beta-ketolase, and accumulated ketocarotenoids. Two major ketocarotenoids were detected, ketolutein and astaxanthin. The level of unesterified astaxanthin reached ca. 14 mu g g(-1) DW in some bkt1 expressing lines of S. phureja but was much lower in the S. tuberosum background. Co-transformation of S. tuberosum with crtB, encoding phytoene synthase, and the bkt1 gene was achieved in order to determine whether this would enhance the levels of S. tuberosum ketocarotenoid. (C) 2006 Elsevier Inc. All rights reserved.