Cell type specificity of neurovascular coupling in cerebral cortex
Cell type specificity of neurovascular coupling in cerebral cortex
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DOI：
10.7554/elife.14315
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发表时间：
2016-05-31
期刊：
影响因子：
7.7
通讯作者：
Devor, Anna
中科院分区：
文献类型：
--
作者：
Uhlirova, Hana;Kilic, Kivilcim;Devor, Anna
识别将神经元活动传递到驱动脑血流动力学的血管系统的细胞参与者和分子信使对于（1）对脑血管调节的基本理解和（2）功能性磁共振成像（fMRI）信号的解释是重要的。在小鼠中使用光遗传学刺激和双光子成像的组合，我们证明了皮质兴奋和抑制的选择性激活激发了不同的血管反应，并将血管收缩机制确定为神经肽Y（NPY）作用于Y1受体。后者意味着在正常生理条件下大脑皮层中与任务相关的负性血氧水平依赖（BOLD）fMRI信号可能主要由NPY阳性抑制性神经元驱动。此外，NPY-Y1通路可能为脑血管疾病提供潜在的治疗靶点。
Identification of the cellular players and molecular messengers that communicate neuronal activity to the vasculature driving cerebral hemodynamics is important for (1) the basic understanding of cerebrovascular regulation and (2) interpretation of functional Magnetic Resonance Imaging (fMRI) signals. Using a combination of optogenetic stimulation and 2-photon imaging in mice, we demonstrate that selective activation of cortical excitation and inhibition elicits distinct vascular responses and identify the vasoconstrictive mechanism as Neuropeptide Y (NPY) acting on Y1 receptors. The latter implies that task-related negative Blood Oxygenation Level Dependent (BOLD) fMRI signals in the cerebral cortex under normal physiological conditions may be mainly driven by the NPY-positive inhibitory neurons. Further, the NPY-Y1 pathway may offer a potential therapeutic target in cerebrovascular disease.