A Water-Soluble, Green-Light Triggered, and Photo-Calibrated Nitric Oxide Donor for Biological Applications
A Water-Soluble, Green-Light Triggered, and Photo-Calibrated Nitric Oxide Donor for Biological Applications
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用于生物应用的水溶性、绿光触发和光校准的一氧化氮供体
DOI：
10.1021/acs.bioconjchem.7b00821
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发表时间：
2018-04-01
影响因子：
4.7
通讯作者：
Yang, Youjun
中科院分区：
文献类型：
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作者：
He, Haihong;Xia, Yang;Yang, Youjun
一氧化氮（NO）是一种多功能的内源性分子，参与多种生理过程，并与许多病理条件的进展有关。因此，NO供体在NO相关的基础和应用中是有价值的工具。传统的自发NO供体在可以监测NO的通量、定位和剂量的情况下是有限的。这促进了新型NO供体的开发，其NO释放不仅是可控的，而且是自校准的。在此，我们报道了一个光触发和光校准的NO供体（NOD 565）与N-亚硝基基团的罗丹明染料。NOD 565不发荧光，在绿色光照射下能有效释放NO。一种明亮的罗丹明染料作为副产物产生，其荧光可用于监测NO的释放。NOD 565在实际应用中的潜力在体外研究中得到了展示，例如，血小板聚集抑制和真菌生长抑制。
Nitric oxide (NO) is a versatile endogenous molecule, involved in various physiological processes and implicated in the progression of many pathological conditions. Therefore, NO donors are valuable tools in NO related basic and applied applications. The traditional spontaneous NO donors are limited in scenarios where flux, localization, and dose of NO could be monitored. This has promoted the development of novel NO donors, whose NO release is not only under control, but also self-calibrated. Herein, we reported a phototriggered and photocalibrated NO donor (NOD565) with an N-nitroso group on a rhodamine dye. NOD565 is nonfluorescent and could release NO efficiently upon irradiation by green light. A bright rhodamine dye is generated as a side-product and its fluorescence can be used to monitor the NO release. The potentials of NOD565 in practical applications are showcased in in vitro studies, e.g., platelet aggregation inhibition and fungi growth suppression.