The effects of locus number, genetic divergence, and genotyping error on the utility of dominant markers for hybrid identification
The effects of locus number, genetic divergence, and genotyping error on the utility of dominant markers for hybrid identification
复制标题
位点数量、遗传分歧和基因分型错误对显性标记在杂交鉴定中的效用的影响
作者：
Michael G. Sovic;L. Kubatko;P. Fuerst
在杂种区的调查中，显性遗传标记通常用于鉴定杂种起源的个体，并将这些个体分配到几个潜在的杂种类别中的一个。定量分析，解决在这样的推理优势标记的统计能力是稀缺的。在这项研究中，显性基因型数据进行了模拟，以评估的影响，第一，分析的基因座的数量，第二，在杂交的组中得分的标记之间的差异的幅度，第三，与数据相关的基因分型错误的水平时，分配个人到各种父母和杂交种类别。分配方法的总体性能在最低水平的分歧检查（Fst <0.4）是相对温和的，但在较高的分化水平（Fst <0.67或0.8）大幅改善。基因分型错误的影响取决于父母类群之间的分歧水平，较大的分歧缓和基因分型错误的影响。这些结果突出了重要性，考虑每个变量的影响时，分配个人的各种父母和杂交类别，并可以帮助指导决策，在未来的杂交研究中采用的位点的数量，以实现所需的功率和分辨率水平。
In surveys of hybrid zones, dominant genetic markers are often used to identify individuals of hybrid origin and assign these individuals to one of several potential hybrid classes. Quantitative analyses that address the statistical power of dominant markers in such inference are scarce. In this study, dominant genotype data were simulated to evaluate the effects of, first, the number of loci analyzed, second, the magnitude of differentiation between the markers scored in the groups that are hybridizing, and third, the level of genotyping error associated with the data when assigning individuals to various parental and hybrid categories. The overall performance of the assignment methods was relatively modest at the lowest level of divergence examined (Fst ˜ 0.4), but improved substantially at higher levels of differentiation (Fst ˜ 0.67 or 0.8). The effect of genotyping error was dependent on the level of divergence between parental taxa, with larger divergences tempering the effects of genotyping error. These results highlight the importance of considering the effects of each of the variables when assigning individuals to various parental and hybrid categories, and can help guide decisions regarding the number of loci employed in future hybridization studies to achieve the power and level of resolution desired.