Monoclonal antibodies specific for porcine monocytes/macrophages: Macrophage heterogeneity in the pig evidenced by the expression of surface antigens
Monoclonal antibodies specific for porcine monocytes/macrophages: Macrophage heterogeneity in the pig evidenced by the expression of surface antigens
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DOI：
10.1111/j.1399-0039.1997.tb02769.x
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发表时间：
1997-04-01
期刊：
影响因子：
--
通讯作者：
Dominquez, J
中科院分区：
文献类型：
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作者：
Bullido, R;delMoral, MG;Dominquez, J
巨噬细胞广泛分布于机体的大部分组织中，在宿主防御和组织损伤修复中发挥重要作用。在这份报告中，我们描述了生产和表征的一组六个单克隆抗体（mAb）对猪巨噬细胞和他们的使用表型组织巨噬细胞。所有mAb均通过用猪肺泡巨噬细胞免疫小鼠产生。其中三个（2A 10/11、3B 11/11和3F 7/11）主要与巨噬细胞反应，并且在较低程度上与血液单核细胞反应，而其他（1 E12/11、2C 12/10和4 E9/11）也识别粒细胞。除了2C 12/10识别的抗原外，这些抗体识别的抗原可以通过Western印迹和/或免疫沉淀来表征。根据它们在还原和非还原条件下的SDS-PAGE中的行为，所有似乎都是单一多肽，其在还原条件下的表观分子量为：1 E12/11和3B 11/11大于204 kDa; 2A 10/11，150 kDa; 4 E9/11，125-170 kDa;和3F 7/11，135 kDa。使用这些单克隆抗体的淋巴和非淋巴器官的免疫组织化学分析揭示了组织巨噬细胞之间的重要抗原异质性。因此，这些单克隆抗体是研究猪巨噬细胞成熟和分化以及确定其在正常和病理条件下的异质性的有用工具。
Macrophages are widely distributed in most tissues of the body, where they play important roles in host defense and repair of tissue damage. In this report we describe the production and characterization of a panel of six monoclonal antibodies (mAb) against porcine macrophages and their use for phenotyping tissue macrophages. All mAbs were produced by immunizing mice with porcine alveolar macrophages. Three of them (2A10/11, 3B1l/11 and 3F7/11) react mainly with macrophages and, at a lower extent, blood monocytes, whereas the others (1E12/11, 2C12/10 and 4E9/11) also recognize granulocytes. Antigens recognized by these antibodies could be characterized by Western blot and/or immunoprecipitation, with the exception of that one recognized by 2C12/10. By their behavior in SDS-PAGE under reducing and nonreducing conditions, all seem to be single polypeptides, whose apparent molecular weight under reducing conditions are:1E12/11 and 3B11/11 larger than 204 kDa; 2A10/11, 150 kDa; 4E9/11, 125-170 kDa; and 3F7/11, 135 kDa. Immunohistochemical analyses of both lymphoid and non-lymphoid organs using these mAbs reveal important antigenic heterogeneity among tissue macrophages. These mAbs are, therefore, useful tools for the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions.