Transcription of the human hsp70 gene is induced by serum stimulation.
Transcription of the human hsp70 gene is induced by serum stimulation.
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人类 hsp70 基因的转录是由血清刺激诱导的。
DOI：
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发表时间：
1985
影响因子：
11.1
通讯作者：
R. Morimoto
中科院分区：
文献类型：
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作者：
Barbara J. Wu;R. Morimoto
我们研究了热休克蛋白（hsp 70）基因在人类细胞中的表达。血清刺激可诱导hsp 70基因的转录和胞浆hsp 70 mRNA的积累。HeLa细胞和人胚肾细胞（细胞系293）的群体是血清饥饿的。在血清刺激后，hsp 70 mRNA的水平在12和18小时之间瞬时增加至10倍高的水平。hsp 70 mRNA水平的增加可以通过hsp 70基因转录速率的10- 15倍增加来解释。当细胞在DNA合成抑制剂1-β-D-阿拉伯呋喃糖基胞嘧啶（araC）存在下进行血清刺激时，hsp 70 mRNA的水平被诱导至不存在抑制剂时检测到的最大水平的仅20%。这表明hsp 70基因的表达与DNA合成偶联。克隆的人热休克蛋白70基因含有调节序列，赋予血清刺激的转录控制。内源性hsp 70基因和含有与细菌氯霉素乙酰转移酶融合的hsp 70基因上游序列的转染嵌合基因都在细胞系293细胞的稳定转染子中暂时表达。内源性hsp 70 mRNA和嵌合mRNA在血清刺激后12-18小时达到最高水平。
We have examined the expression of the heat shock protein (hsp70) gene in human cells. The transcription of the hsp70 gene and accumulation of cytoplasmic hsp70 mRNA is induced by serum stimulation. Populations of HeLa cells and human embryonic kidney cells (cell line 293) were serum starved. Upon serum stimulation, the level of hsp70 mRNA transiently increases between 12 and 18 hr to a 10-fold higher level. The increased levels of hsp70 mRNA can be accounted for by a 10- to 15-fold increase in the rate of transcription of the hsp70 gene. When cells were serum-stimulated in the presence of an inhibitor of DNA synthesis, 1-beta-D-arabinofuranosylcytosine (araC), the levels of hsp70 mRNA were induced to only 20% of the maximal level detected in the absence of the inhibitor. This suggests that the expression of the hsp70 gene is coupled to DNA synthesis. The cloned human hsp70 gene contains regulatory sequences that confer serum-stimulated transcriptional control. The endogenous hsp70 gene and the transfected chimeric gene containing sequences upstream of the hsp70 gene fused to bacterial chloramphenicol acetyltransferase are both temporally expressed in stable transfectants of cell line 293 cells. The endogenous hsp70 mRNA and the chimeric mRNA reach maximum levels 12-18 hr after serum stimulation.