Magnetic graphene-based nanotheranostic agent for dual-modality mapping guided photothermal therapy in regional lymph nodal metastasis of pancreatic cancer
Magnetic graphene-based nanotheranostic agent for dual-modality mapping guided photothermal therapy in regional lymph nodal metastasis of pancreatic cancer
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基于磁性石墨烯的纳米治疗剂用于胰腺癌区域淋巴结转移的双模态标测引导光热治疗
DOI：
10.1016/j.biomaterials.2014.07.064
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发表时间：
2014-11-01
期刊：
影响因子：
14
通讯作者：
Fu, De L.
中科院分区：
文献类型：
--
作者：
Wang, Sheng;Zhang, Qin;Fu, De L.
尽管区域淋巴结清扫仍然是治愈胰腺癌转移的唯一方法，但它不可避免地伴随着较大的创伤、多种并发症和较低的手术切除率。因此，探索消融耐药胰腺癌的治疗方法一直备受关注。此外，治疗期间术后和术中 RLN 的标测也很重要，因为肉眼只能检测到少数淋巴结。在我们的研究中，氧化铁纳米粒子（GO-IONP）修饰的氧化石墨烯作为纳米治疗剂首次被开发用于诊断和治疗胰腺癌的RLN转移。该方法是根据临床实践设计的，将GO-IONP药剂直接注射到肿瘤中，通过淋巴管转运至RLN。与目前临床手术中使用的商业碳纳米颗粒相比，GO-IONP显示出通过磁共振成像（MRI）对区域淋巴系统进行双模态映射的强大能力，并且试剂的深色提供了有价值的信息，有助于外科医生在术前制定术前计划以及术中区分RLN与周围组织。在双模态标测的指导下，我们进一步证明了通过切口手术的近红外（NIR）照射可以有效消融包括腹部淋巴结在内的转移淋巴结。我们的动物实验也清楚地说明了 GO-IONP 较低的系统毒性和光热疗法 (FIT) 对邻近组织的安全性。使用 GO-IONP 作为纳米治疗剂提供了一种对 RLN 进行标测和光热消融的方法，后者可以作为侵入性手术淋巴结清扫的替代方法。 (C) 2014 Elsevier Ltd. 保留所有权利。
Although regional lymph nodes (RLN) dissection remains the only way to cure pancreatic cancer metastasis, it is unavoidably associated with sizable trauma, multiple complications, and low surgical resection rates. Thus, exploring a treatment approach for the ablation of drug-resistant pancreatic cancer is always of great concern. Moreover, reoperative and intraoperative mapping of RLN is also important during treatment, because only a few lymph nodes can be detected by the naked eye. In our study, graphene oxides modified with iron oxide nanoparticles (GO-IONP) as a nanotheranostic agent is firstly developed to diagnose and treat RLN metastasis of pancreatic cancer. The approach was designed based on clinical practice, the GO-IONP agent directly injected into the tumor was transported to RLN via lymphatic vessels. Compared to commercial carbon nanoparticles currently used in the clinic operation, the GO-IONP showed powerful ability of dual-modality mapping of regional lymphatic system by magnetic resonance imaging (MRI), as well as dark color of the agent providing valuable information that was instrumental for surgeon in making the preoperative plan before operation and intraoperatively distinguish RLN from surrounding tissue. Under the guidance of dual-modality mapping, we further demonstrated that metastatic lymph nodes including abdominal nodes could be effectively ablated by near-infrared (NIR) irradiation with an incision operation. The lower systematic toxicity of GO-IONP and satisfying safety of photothermal therapy (FIT) to neighbor tissues have also been clearly illustrated in our animal experiments. Using GO-IONP as a nanotheranostic agent presents an approach for mapping and photothermal ablation of RLN, the later may serve as an alternative to lymph node dissection by invasive surgery. (C) 2014 Elsevier Ltd. All rights reserved.