Quantitative measurement of HCV RNA in the serum: A comparison of three assays based on different principles
Quantitative measurement of HCV RNA in the serum: A comparison of three assays based on different principles
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血清中 HCV RNA 的定量测量：基于不同原理的三种检测方法的比较
DOI：
10.1111/j.1440-1746.1997.tb00473.x
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发表时间：
1997
影响因子：
4.1
通讯作者：
E. Tanaka
中科院分区：
文献类型：
--
作者：
Tetsuya Ichijo;A. Matsumoto;Masakazu Kobayashi;K. Furihata;E. Tanaka
丙型肝炎病毒（HCV）RNA的定量测量是有用的慢性丙型肝炎患者，特别是干扰素治疗。我们通过将其与其他两种具有不同原理的检测方法进行比较，研究了AMPLOYCOR监测检测方法（一种新开发的定量检测方法）的临床实用性。共研究了48例接受干扰素-α（IFN-α）治疗的慢性丙型肝炎患者：19例完全应答者，29例无应答者。通过AMPLICATION COR、分支DNA（bDNA）探针和竞争性聚合酶链反应（C-PCR）测定定量测定丙型肝炎病毒RNA。在AMPLICATCOR测定中使用内部定量标准品。在C-PCR方法中使用在中间部分缺失15个碱基对的cDNA竞争物。HCVRNA浓度在本研究中采用的三种检测方法之间具有显著相关性。C-PCR检测的灵敏度为100%，AMPDECOR检测的灵敏度为90%，bDNA检测的灵敏度为69%。C-PCR检测的活性定量范围最好，bDNA检测的活性定量范围最差。与其他两种检测相比，bDNA检测在血清2型患者中显示出较低的值。在开始IFN-α治疗前，所有三种试验中IFN-α治疗长期应答的预测率均超过75%。通过C-PCR和AMPDECOR检测，完成IFN-α治疗后的预测率高达80%，但bDNA检测的预测率较低（58%）。bDNA和AMPLICOR检测试剂盒的处理比C-PCR检测试剂盒容易得多，后者需要时间和技能。结果表明，该方法是一种简便、可靠的血清HCVRNA检测方法，适合临床应用。
Quantitative measurement of hepatitis C virus (HCV) RNA is useful in patients with chronic hepatitis C, especially with interferon treatment. We examined the clinical usefulness of the AMPLICOR monitor assay, a newly developed assay for quantitative measurement, by comparing it with two other assays with different principles. A total of 48 patients with chronic hepatitis C who were treated with interferon‐α (IFN‐α) were studied: 19 were complete responders and 29 were non‐responders. Hepatitis C virus RNA was measured quantitatively by AMPLICOR, branched DNA (bDNA) probe, and competitive polymerase chain reaction (C‐PCR) assays. An internal quantification standard was used in the AMPLICOR assay. A cDNA competitor with a deletion of 15 base pairs in the middle portion was used in the C‐PCR method. The concentration of HCV RNA was significantly correlated between the three assays adopted in this study. Sensitivity of assays was 100% by C‐PCR, 90% by AMPLICOR and 69% by bDNA assays. The active quantitative range was best with the C‐PCR assay and worst with the bDNA assay. The bDNA assay had a tendency to exhibit lower values for patients with serotype 2 than did the other two assays. The predictive rate of the long‐term response to IFN‐α therapy, before its initiation, was over 75% in all three assays. The predictive rate just after completing IFN‐α therapy was as high as 80% by C‐PCR and the AMPLICOR assays, but was low (58%) with the bDNA assay. The handling of the bDNA and AMPLICOR assays was much easier than the C‐PCR assay, which required time and skill. These results indicate that the AMPLICOR assay is a simple and reliable method for measuring the serum concentrations of HCV RNA, and thus is suitable for clinical application.
影响因子：
2.9
作者：
MYERS, RA;ZAFARALLA, GC;OLIVERA, BM
通讯作者：
OLIVERA, BM