Malaria associated apoptosis is not significantly correlated with either parasitemia or the number of previous malaria attacks
Malaria associated apoptosis is not significantly correlated with either parasitemia or the number of previous malaria attacks
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DOI：
10.1007/s00436-002-0816-z
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发表时间：
2003-05-01
影响因子：
2
通讯作者：
Ferreira-da-Cruz, MD
中科院分区：
文献类型：
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作者：
Riccio, EKP;Neves, I;Ferreira-da-Cruz, MD
本文对79例非合并症恶性疟原虫或间日疟原虫门诊患者和30例健康人血液标本中淋巴细胞凋亡的发生率和强度进行了研究。在分析离体淋巴细胞时，健康个体和疟疾患者之间的细胞凋亡百分比没有差异。然而，当细胞培养24小时后，恶性疟原虫和间日疟原虫感染患者的淋巴细胞凋亡水平均显著升高。CD4（+）和CD8(+) T细胞在恶性疟原虫和间日疟原虫感染患者中受到的影响程度相当。然而，当我们比较感染和未感染个体的细胞凋亡值时，CD4(+) T细胞似乎比CD8(+) T细胞更容易受到影响：与健康个体相比，疟疾患者血浆中sIL-2R水平显著增加，感染患者的sIL-2R水平与细胞凋亡率呈正相关，细胞凋亡率增加。经恶性疟原虫抗原或抗cd3单克隆抗体刺激后，Fas抗原表达增加。这些数据表明，在疟疾感染期间，有一定比例的淋巴细胞死于凋亡，体内活化的细胞在体外表达凋亡过程需要一段时间。
The occurrence and intensity of lymphocyte apoptosis in blood samples from 79 outclinic patients with uncomplicated Plasmodium falciparum or Plasmodium vivax malaria and 30 healthy individuals were investigated. No difference in apoptosis percentages was detected between healthy individuals and malaria patients when ex vivo lymphocytes were analyzed. However, significantly increased apoptosis levels were observed in lymphocytes from both P. falciparum and P. vivax-infected patients when the cells were cultured for 24 h. CD4(+) and CD8(+) T cells were affected to a comparable extent in P.falciparum- and P.vivax-infected patients. However, when we compared apoptosis values in infected and non-infected individuals it appeared that CD4(+) T cells were more susceptible than CD8(+) T cells: A significant increase in the sIL-2R plasma levels was observed in malaria patients when compared with healthy individuals and a positive correlation was observed between sIL-2R levels and apoptosis rates in infected patients presenting increased rates of apoptosis. An increased expression of Fas antigen was recorded after stimulation with P. falciparum antigen or anti-CD3 monoclonal antibody. These data show that a consistent proportion of the lymphocyte population dies by apoptosis during a malaria infection and that a period of time is necessary before in vivo activated cells can express the apoptotic process in vitro.