Proton nuclear magnetic resonance measurement of the amide hydrogen exchange rates of group A streptococcal polysaccharide in H2O.
Proton nuclear magnetic resonance measurement of the amide hydrogen exchange rates of group A streptococcal polysaccharide in H2O.
复制标题
质子核磁共振测量 A 族链球菌多糖在水中的酰胺氢交换率。
DOI：
10.1016/0006-291x(87)91408-2
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Krishna,NR
中科院分区：
文献类型：
--
作者：
Huang,DH;Pritchard,DG;Sakai,TT;Krishna,NR
The solvent exchange rates of the acetamido hydrogen of the 2-acetamido-2-deoxy-β-D-glucopyranosyl unit of group A streptococcal polysaccharide dissolved in H 2 O have been measured and compared with the corresponding exchange rates in the solvated model compound 1-O-methyl-2-acetamido-2-deoxy-β-D-glucopyranoside. Amide hydrogen exchange rates were measured at 25° C over a wide pH range by a combination of two separate NMR techniques:(i) the transfer of solvent saturation and (ii) the amide hydrogen saturation recovery NMR experiments. The data indicate that the acetamido hydrogen essentially exists in a solvated environment and that its contribution to the conformational stability of this polysaccharide through intramolecular hydrogen bonding is negligible.
