A CLE-BAM-CIK signalling module controls root protophloem differentiation in Arabidopsis
A CLE-BAM-CIK signalling module controls root protophloem differentiation in Arabidopsis
复制标题
CLE-BAM-CIK 信号模块控制拟南芥根原韧皮部分化。
DOI：
10.1111/nph.17791
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发表时间：
2021-10-27
期刊：
影响因子：
9.4
通讯作者：
Gou, Xiaoping
中科院分区：
文献类型：
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作者：
Hu, Chong;Zhu, Yafen;Gou, Xiaoping
外源应用 CLAVATA3 (CLV3)/胚胎周围区域 (CLE) 肽会抑制原韧皮部分化并导致近端根分生组织的消耗。然而，确切的CLE肽和相应的调节原韧皮部分化的受体复合物尚未阐明。通过表达模式和系统发育分析，CLE25/26/45被鉴定为候选肽。进一步的遗传分析、生理测定和特定的原韧皮部标记观察表明，CLE25/26/45、几乎没有 MERISTEM1/3 (BAM1/3) 和 CLV3 不敏感激酶 (CIK) 参与调节原韧皮部分化。 cle25 26 45和cik2 3 4 5 6突变可以极大地挽救短短根（brx）和章鱼（ops）突变体的根部缺陷。 clv1 bam1 3 和 cik2 3 4 5 6 的原韧皮部分化和近端根分生组织消耗对 CLE25/26/45 处理不敏感。 cle25 26 45、clv1 bam1 3 和 cik2 3 4 5 6 显示出类似的早熟原韧皮部。此外，CLE25/26/45在体内诱导BAM和CIK之间的相互作用。此外，CLE25/26/45 增强了 CIK 的磷酸化水平，而在 clv1 bam1 3 突变体中，CIK 的磷酸化水平大大受损。我们的工作阐明，CLE25/26/45-BAM1/3-CIK2/3/4/5/6 信号模块在 BRX 和 OPS 下游遗传作用，抑制原韧皮部分化。
Exogenous application of CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGION (CLE) peptides suppresses protophloem differentiation and leads to the consumption of the proximal root meristem. However, the exact CLE peptides and the corresponding receptor complex regulating protophloem differentiation have not yet been clarified. Through expression pattern and phylogenetic analyses, CLE25/26/45 were identified as candidate peptides. Further genetic analyses, physiological assays and specific protophloem marker observations indicated that CLE25/26/45, BARELY ANY MERISTEM1/3 (BAM1/3) and CLV3 INSENSITIVE KINASEs (CIKs) are involved in regulating protophloem differentiation. The cle25 26 45 and cik2 3 4 5 6 mutation can greatly rescue the root defects of brevis radix (brx) and octopus (ops) mutants. The protophloem differentiation and proximal root meristem consumption of clv1 bam1 3 and cik2 3 4 5 6 were insensitive to CLE25/26/45 treatments. cle25 26 45, clv1 bam1 3 and cik2 3 4 5 6 displayed similar premature protophloem. In addition, CLE25/26/45 induced the interactions between BAMs and CIKs in vivo. Furthermore, CLE25/26/45 enhanced the phosphorylation levels of CIKs, which were greatly impaired in clv1 bam1 3 mutant. Our work clarifies that the CLE25/26/45-BAM1/3-CIK2/3/4/5/6 signalling module genetically acts downstream of BRX and OPS to suppress protophloem differentiation.