Prevalent human coxsackie B-5 virus infects porcine islet cells primarily using the coxsackie-adenovirus receptor.
Prevalent human coxsackie B-5 virus infects porcine islet cells primarily using the coxsackie-adenovirus receptor.
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流行的人类柯萨奇 B-5 病毒主要利用柯萨奇腺病毒受体感染猪胰岛细胞。
DOI：
10.1111/j.1399-3089.2004.00183.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Njenga,MKariuki
中科院分区：
文献类型：
--
作者：
Myers,SuzanneE;Brewer,Laurie;Shaw,DanielP;Greene,WallaceH;Love,BrendaC;Hering,Bernhard;Spiller,OBrad;Njenga,MKariuki
Abstract:Background:We have previously demonstrated that transplanting porcine encephalomyocarditis virus (EMCV)‐infected porcine islet cells (PICs) results in transmission of the virus to recipient mice, which is manifested by acute fatal infection within 5 to 8 days. Here, we determined PIC susceptibility to a related and highly prevalent human picornavirus, coxsackie B‐5 virus (CVB‐5).Methods:PICs were inoculated with CVB‐5 in vitro for up to 96 hours and infectivity, level of virus replication, and cellular function determined. Subsequently, monoclonal and polyclonal antibody blocking experiments were used to investigate the receptor CVB‐5 uses to enter PICs, and the ability of CVB‐5‐infected islets to reverse diabetes analyzed in mice.Results:Adult pig islets inoculated with CVB‐5 in vitro showed a typical picornaviral replication cycle with a 2‐h lag phase followed by a 4‐h exponential phase during which the virus titer increased by 4 logs. However, CVB‐5 was less cytolytic to PICs than EMCV, resulting in a persistent productive infection lasting for up to 96 h, with minimal evidence of cell lysis. Double immunostaining confirmed the presence of CVB‐5 antigens in insulin‐producing islets. Infection of PICs in the presence of antibodies against human coxsackie‐adenovirus receptor (CAR) resulted in near complete blockage in production of infectious virus particles whereas blocking with anti‐porcine decay‐accelerating factor (DAF, also called CD55) or anti‐porcine membrane cofactor protein (MCP, also called CD46) only slightly decreased the number of infectious CVB‐5 particles produced. Immunofluoresence staining showed CAR and MCP expression on the islet surface, but not DAF. Transplanting CVB‐5‐infected PICs into diabetic C57BL/6 mice resulted in reversal of diabetes.Conclusion:Although PICs are susceptible to human CVB‐5, the infection does not appear to affect xenograft function in vitro or in vivo in the short term.
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影响因子：
4.4
作者：
T. Yamamoto;C. Wilson
通讯作者：
T. Yamamoto;C. Wilson
DOI：
10.1084/jem.149.2.448
发表时间：
1979-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Biesecker G;Podack ER;Halverson CA;Müller-Eberhard HJ
通讯作者：
Müller-Eberhard HJ
海曼肾炎的免疫发病机制。
DOI：
10.1016/0090-1229(84)90304-0
发表时间：
1984
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Biesecker,G;Noble,B;Andres,GA;Koffler,D
通讯作者：
Koffler,D
在补体缺失的情况下，被动转移的抗刷状缘抗体会诱导近端小管损伤。
影响因子：
4.6
作者：
Noble,B;Andres,GA;Brentjens,JR
通讯作者：
Brentjens,JR
影响因子：
15.9
作者：
SALANT, DJ;BELOK, S;COUSER, WG
通讯作者：
COUSER, WG