Differential expression of CD22 (Lyb8) on murine B cells
Differential expression of CD22 (Lyb8) on murine B cells
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DOI：
10.1093/intimm/8.7.1121
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发表时间：
1996-07-01
影响因子：
4.4
通讯作者：
Waldschmidt, TJ
中科院分区：
文献类型：
--
作者：
Erickson, LD;Tygrett, LT;Waldschmidt, TJ
以前的研究已经确定了人CD 22（一种B细胞限制性糖蛋白）的分布、生物化学和功能属性。最近，小鼠CD 22等价物的分子克隆揭示了该分子与先前描述的Lyb 8同种异体抗原相同。使用抗Lyb 8 mAb Cy34.1.2，本报告记录了小鼠B细胞区室中CD 22的表达模式。结果表明，在骨髓中，鼠CD 22在前B细胞、前B细胞和新出现的IgM（+）B细胞的表面上不存在。CD22以低密度存在于未成熟IgM（hi）B细胞上，并在成熟再循环B细胞上完全表达。在外周，鼠CD 22在所有B细胞亚群上以成熟水平表达，包括滤泡、边缘区、B1和转换B细胞。进一步的研究表明，CD 22在活化的鼠B细胞上保留较长的时间。最后，结合CD 23和热稳定抗原，CD 22可用于描述未成熟的脾B细胞，并将其与滤泡和边缘区细胞区分开来。总之，结果证明鼠CD 22是所有成熟B细胞亚群的有用的泛标志物。
Previous studies have established the distribution, biochemistry and functional attributes of human CD22, a B cell-restricted glycoprotein. Recently, molecular cloning of the murine CD22 equivalent revealed this molecule to be the same as the previously described Lyb8 alloantigen. Using the anti-Lyb8 mAb Cy34.1.2, the present report documents the expression patterns of CD22 within the murine B cell compartment. The results demonstrate that in the bone marrow, murine CD22 is absent on the surface of pro-B cells, pre-B cells and newly emerging IgM(+) B cells. CD22 is present at a low density on immature IgM(hi) B cells and fully expressed on mature recirculating B cells. In the periphery, murine CD22 is expressed at mature levels on all B cell subsets including follicular, marginal zone, B1 and switched B cells. Further studies showed CD22 to be retained on activated murine B cells for extended periods. Finally, in combination with CD23 and heat stable antigen, CD22 can be used to delineate the immature splenic B cells, and distinguish them from follicular and marginal zone cells. Together, the results demonstrate murine CD22 to be a useful pan marker for all mature B cell subsets.