Occurrence of cell surface arabinogalactan-protein and extensin epitopes in relation to pericycle and vascular tissue development in the root apex of four species
Occurrence of cell surface arabinogalactan-protein and extensin epitopes in relation to pericycle and vascular tissue development in the root apex of four species
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DOI：
10.1007/s004250050254
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发表时间：
1998-02-01
期刊：
影响因子：
4.3
通讯作者：
Knox, JP
中科院分区：
文献类型：
--
作者：
Casero, PJ;Casimiro, I;Knox, JP
识别两类发育调节植物细胞表面成分 - 阿拉伯半乳聚糖蛋白 (AGP) 和延伸蛋白 - 的单克隆抗体已用于免疫标记具有不同中柱鞘和维管组织发育特征的四个物种的根尖细胞。使用抗 AGP 单克隆抗体 JIM4 和 JIM13 以及抗伸展蛋白单克隆抗体 JIM11、JIM12、JIM19 和 JIM20 对豌豆 (Pisum sativum L.)、萝卜 (Raphanus sativus L.)、胡萝卜 (Daucus carota L.) 和洋葱 (Allium cepa L.) 的根尖进行免疫标记。所有这些抗体都识别这些物种中至少一种（但不是全部）的中柱鞘细胞亚群。表位出现的限制模式也反映了血管细胞的发育。讨论了四个物种中抗体识别模式的差异以及这些细胞表面分子在细胞分化和根模式事件中的可能作用。
Monoclonal antibodies recognizing two classes of developmentally regulated plant cell surface components - arabinogalactan-proteins (AGPs) and extensins - have been used to immunolabel cells at the root apices of four species with different characteristics of pericycle and vascular tissue development. Root apices of pea (Pisum sativum L.), radish (Raphanus sativus L.), carrot (Daucus carota L.) and onion (Allium cepa L.) were immunolabled with the anti-AGP monoclonal antibodies JIM4 and JIM13 and anti-extensin monoclonal antibodies JIM11, JIM12, JIM19 and JIM20. All of these antibodies recognized subsets of pericycle cells in at least one, but never all, of these species. The restricted patterns of epitope occurrence also reflected vascular cell development. The differences in patterns of antibody recognition in the four species are discussed in relation to the possible roles of these cell surface molecules in cell differentiation and root patterning events.