Mechanical Trap Surface-Enhanced Raman Spectroscopy for Three-Dimensional Surface Molecular Imaging of Single Live Cells
Mechanical Trap Surface-Enhanced Raman Spectroscopy for Three-Dimensional Surface Molecular Imaging of Single Live Cells
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DOI：
10.1002/anie.201700695
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发表时间：
2017-03-27
影响因子：
16.6
通讯作者：
Gracias, David H.
中科院分区：
文献类型：
--
作者：
Jin, Qianru;Li, Ming;Gracias, David H.
报道了一种新的基于壳层的光谱平台，命名为机械陷阱表面增强拉曼光谱（MTSERS），用于同时捕获，分析和三维显微镜映射单个活细胞膜上的内在分子特征。通过利用等离子体金纳米星对MT内表面的功能化以及细胞膜的共形接触，MTSERS可以实现出色的信号增强，可靠地检测分子特征，并允许分析物的非微扰、多重3D表面成像，例如单细胞表面的脂质和蛋白质。所展示的能力强调了MTSERS进行3D光谱显微成像和提供活细胞群中生物学可解释的、定量的和动态的分子图谱的潜力。
Reported is a new shell-based spectroscopic platform, named mechanical trap surface-enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on the membrane of single live cells. By leveraging the functionalization of the inner surfaces of the MTs with plasmonic gold nanostars, and conformal contact of the cell membrane, MTSERS permits excellent signal enhancement, reliably detects molecular signatures, and allows non-perturbative, multiplex 3D surface imaging of analytes, such as lipids and proteins on the surface of single cells. The demonstrated ability underscores the potential of MTSERS to perform 3D spectroscopic microimaging and to furnish biologically interpretable, quantitative, and dynamic molecular maps in live cell populations.