MicroRNA-155 inhibits the osteogenic differentiation of mesenchymal stem cells induced by BMP9 via downregulation of BMP signaling pathway.
MicroRNA-155 inhibits the osteogenic differentiation of mesenchymal stem cells induced by BMP9 via downregulation of BMP signaling pathway.
复制标题
MicroRNA-155通过下调BMP信号通路抑制BMP9诱导的间充质干细胞成骨分化
DOI：
10.3892/ijmm.2018.3526
复制
发表时间：
2018-06
影响因子：
5.4
通讯作者：
Weng Y
中科院分区：
文献类型：
--
作者：
Liu H;Zhong L;Yuan T;Chen S;Zhou Y;An L;Guo Y;Fan M;Li Y;Sun Y;Li W;Shi Q;Weng Y
Previous studies have indicated that bone morphogenetic protein 9 (BMP9) can promote the osteogenic differentiation of mesenchymal stem cells (MSCs) and increase bone formation in bone diseases. However, the mechanisms involved remained poorly understood. It is necessary to investigate the specific regulatory mechanisms of osteogenic differentiation that were induced by BMP9. During the process of osteogenic differentiation induced by BMP9, the expression of microRNA-155 (miR-155) exhibited a tendency of increasing at first and then decreasing, which made us consider that miR-155 may have a modulatory role in this process, but the roles of this process have not been elucidated. This study aimed to uncover miR-155 capable of concomitant regulation of this process. mmu-miR-155 mimic (miR-155) was transfected into MSCs and osteogenesis was induction by using recombinant adenovirus expressing BMP9. Overexpressed miR-155 in MSCs led to a decrease in alkaline phosphatase (ALP) staining and Alizarin red S staining during osteogenic differentiation, and reduced the expression of osteogenesis-related genes, such as runt-related transcription factor 2 (Runx2), osterix (OSX), osteocalcin (OCN) and osteopontin (OPN). On protein levels, overexpressed miR-155 markedly decreased the expression of phosphorylated Smad1/5/8 (p-Smad1/5/8), Runx2, OCN and OPN. Luciferase reporter assay revealed Runx2 and bone morphogenetic protein receptor 9 (BMPR2) are two direct target genes of miR-155. Downregulation of the expression of Runx2 and BMPR2, respectively could offset the inhibitory effect of miR-155 in the osteogenesis of MSCs. In vivo, subcutaneous ectopic osteogenesis of MSCs in nude mice showed miR-155 inhibited osteogenic differentiation. In conclusion, our results demonstrated that miR-155 can inhibit the osteogenic differentiation induced by BMP9 in MSCs.
登录
查看更多内容
影响因子：
9
作者：
通讯作者：
--
影响因子：
2.8
作者：
Luu, Hue H.;Song, Wen-Xin;He, Tong-Chuan
通讯作者：
He, Tong-Chuan
影响因子：
4.8
作者：
Brown, MA;Zhao, QH;Choe, S
通讯作者：
Choe, S
影响因子：
--
作者：
Qi, Renli;Long, Dingbiao;Huang, Jinxiu
通讯作者：
Huang, Jinxiu
由稳定线产生的含prodomain的BMP9有效地调节了间充质干细胞的分化。
影响因子：
3.6
作者：
Li R;Yan Z;Ye J;Huang H;Wang Z;Wei Q;Wang J;Zhao L;Lu S;Wang X;Tang S;Fan J;Zhang F;Zou Y;Song D;Liao J;Lu M;Liu F;Shi LL;Athiviraham A;Lee MJ;He TC;Zhang Z
通讯作者：
Zhang Z