Immunological responses of mice following administration of natural rubber latex proteins by different routes of exposure.
Immunological responses of mice following administration of natural rubber latex proteins by different routes of exposure.
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通过不同的暴露途径给予天然橡胶乳胶蛋白后小鼠的免疫反应。
DOI：
10.1093/toxsci/55.2.343
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Meade,BJ
中科院分区：
文献类型：
--
作者：
Woolhiser,MR;Munson,AE;Meade,BJ
虽然IgE介导的乳胶过敏的患病率在过去十年中有所增加，但最终导致致敏的情况仍不确定。这些研究的目的是评价致敏途径在乳胶过敏发生中的作用，使用代表可能致敏的潜在暴露途径（医护人员（局部和呼吸道）和脊柱裂患者（皮下））的鼠模型。BALB/c小鼠通过皮下、局部、鼻内或鼻内途径给予乳胶蛋白后，其总IgE呈剂量反应性升高。体外脾细胞刺激最初证明了小鼠对乳胶蛋白免疫应答的特异性。随后，使用免疫印迹分析比较致敏途径中乳胶特异性IgE的产生。从皮下致敏小鼠的IgE的免疫印迹表明识别乳胶蛋白的分子量接近14 kDa和27 kDa。这些蛋白质的大小与主要乳胶过敏原（Hev b1和Hev b3）的分子量一致，高比例的脊柱裂患者产生抗体。致敏小鼠intrichelheal或局部给药表现出联合IgE识别乳胶蛋白近14 kDa，35 kDa，和92 kDa。这些分子量与其他乳胶过敏原（Hev b6，Hev b2和Hev b4）相似，通常由医护人员的IgE识别。通过局部、鼻内或鼻内暴露对乳胶蛋白致敏的小鼠在乳胶蛋白呼吸激发后通过全身体积描记法评价表现出支气管收缩。亚稳态致敏小鼠无反应。四种不同致敏途径中乳胶特异性IgE免疫印迹特征和肺功能改变的这些差异表明，导致致敏的暴露途径可能在确定主要过敏原和过敏反应的临床表现中发挥作用。
Although the prevalence of IgE-mediated latex allergy has increased over the past decade, the circumstances which culminate in sensitization remain uncertain. The objective of these studies was to evaluate the role which sensitization route plays in the development of latex allergy using murine models representative of potential exposure routes by which health care workers (topical and respiratory) and spina bifida patients (subcutaneous) may be sensitized. BALB/c mice administered latex proteins by the subcutaneous, topical, intranasal, or intratracheal routes exhibited dose-responsive elevations in total IgE.In vitrosplenocyte stimulation initially demonstrated specificity of the murine immune response to latex proteins. Subsequently, immunoblot analysis was used to compare latex-specific IgE production amongst sensitization routes. Immunoblots of IgE from subcutaneously sensitized mice demonstrated recognition of latex proteins with molecular weights near 14 kDa and 27 kDa. These protein sizes are consistent with the molecular weights of major latex allergens (Hev b1 andHev b3), to which high percentages of spina bifida patients develop antibodies. Mice sensitized by intratracheal or topical administration exhibited combined IgE recognition of latex proteins near 14 kDa, 35 kDa, and 92 kDa. These molecular weights are similar to other latex allergens (Hev b6,Hev b2, andHev b4) commonly recognized by IgE of health care workers. Mice sensitized to latex proteins by topical, intranasal, or intratracheal exposures exhibited bronchoconstriction as evaluated by whole body plethysmography following respiratory challenge with latex proteins. Subcutaneously sensitized mice were unresponsive. These differences in latex-specific IgE immunoblot profiles and altered pulmonary function amongst the four different sensitization routes suggest that exposure routes leading to sensitization may play a role in determining the primary allergen(s), and the clinical manifestation of the allergic responses.