Author Correction: Injectable tissue integrating networks from recombinant polypeptides with tunable order.
Author Correction: Injectable tissue integrating networks from recombinant polypeptides with tunable order.
复制标题
作者更正：可注射组织整合来自具有可调顺序的重组多肽的网络。
DOI：
10.1038/s41563-018-0233-z
复制
发表时间：
2018
期刊：
影响因子：
41.2
通讯作者：
Chilkoti,Ashutosh
中科院分区：
文献类型：
--
作者：
Roberts,Stefan;Harmon,TylerS;Schaal,JeffreyL;Miao,Vincent;Li,KanJonathan;Hunt,Andrew;Wen,Yi;Oas,TerrenceG;Collier,JoelH;Pappu,RohitV;Chilkoti,Ashutosh
天然生物材料的新特性是有序和无序结构域之间纳米尺度相互作用的集体效应的结果。在这里，利用重组序列设计，我们创造了一组偏序多肽，通过精确编码纳米级的有序-无序相互作用来研究出现的层级结构。这些材料结合了无序弹性蛋白样多肽的刺激响应性和聚丙氨酸螺旋的结构稳定性，具有可调的热滞和在阈值温度以上可逆形成多孔粘弹性网络的能力。通过粗粒模拟，我们证明了磁滞现象是由于有序和无序微区的介观相分离引起的物理交联引起的。在注射部分有序的多肽后，它们可以在体温下转变，形成稳定的多孔支架，快速整合到周围组织中，炎症最小，血管形成程度高。结构有序性和无序性的序列水平调控是基于蛋白质的生物功能材料设计中尚未开发的原理。
Emergent properties of natural biomaterials result from the collective effects of nanoscale interactions among ordered and disordered domains. Here, using recombinant sequence design, we have created a set of partially ordered polypeptides to study emergent hierarchical structures by precisely encoding nanoscale order–disorder interactions. These materials, which combine the stimuli-responsiveness of disordered elastin-like polypeptides and the structural stability of polyalanine helices, are thermally responsive with tunable thermal hysteresis and the ability to reversibly form porous, viscoelastic networks above threshold temperatures. Through coarse-grain simulations, we show that hysteresis arises from physical crosslinking due to mesoscale phase separation of ordered and disordered domains. On injection of partially ordered polypeptides designed to transition at body temperature, they form stable, porous scaffolds that rapidly integrate into surrounding tissue with minimal inflammation and a high degree of vascularization. Sequence-level modulation of structural order and disorder is an untapped principle for the design of functional protein-based biomaterials.