The postsynaptic density protein, IQ-ArfGEF/BRAG1, can interact with IRSp53 through its proline-rich sequence
The postsynaptic density protein, IQ-ArfGEF/BRAG1, can interact with IRSp53 through its proline-rich sequence
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DOI：
10.1016/j.brainres.2008.11.061
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发表时间：
2009-01-28
期刊：
影响因子：
2.9
通讯作者：
Sakagami, Hiroyuki
中科院分区：
文献类型：
--
作者：
Sanda, Masashi;Kamata, Akifumi;Sakagami, Hiroyuki
IQ-ArfGEF/BRAG 1是Arf 1和Arf 6的鸟嘌呤核苷酸交换因子，定位于突触后密度（PSD）并与PSD-95相互作用。在这项研究中，我们确定了一种新的相互作用IQ-ArfGEF/BRAG 1与胰岛素受体酪氨酸激酶底物53 kDa（IRSp 53），也被称为脑特异性血管生成抑制因子1相关蛋白2。这种相互作用是由IQArfGEF/BRAG 1的C-末端富含脯氨酸的序列与IRSp 53的SH 3结构域的结合介导的。IQ-ArfGEF/BRAG 1和IRSp 53共定位于某些神经元群体的兴奋性突触的PSD。我们目前的研究结果表明，IQ-ArfGEF/BRAG 1可能通过与多价PSD蛋白如IRSp 53和PSD-95的相互作用在NMDA受体下游发挥作用。(c)2008 Elsevier B. V.保留所有权利。
IQ-ArfGEF/BRAG1, a guanine nucleotide exchange factor for Arf1 and Arf6, is localized at the postsynaptic density (PSD) and interacts with PSD-95. In this study, we identified a novel interaction of IQ-ArfGEF/BRAG1 with insulin receptor tyrosine kinase substrate of 53 kDa (IRSp53), also known as brain-specific angiogenesis inhibitor 1-associated protein 2. The interaction was mediated by the binding of the C-terminal proline-rich sequence of IQArfGEF/BRAG1 to the SH3 domain of IRSp53. IQ-ArfGEF/BRAG1 and IRSp53 were colocalized at the PSD of excitatory synapses of certain neuronal populations. Our present findings suggest that IQ-ArfGEF/BRAG1 may play roles downstream of NMDA receptors through the interaction with multivalent PSD proteins such as IRSp53 and PSD-95. (c) 2008 Elsevier B.V. All rights reserved.