MONOCLONAL-ANTIBODY TO HUMAN ACUTE LYMPHOBLASTIC-LEUKEMIA ANTIGEN
MONOCLONAL-ANTIBODY TO HUMAN ACUTE LYMPHOBLASTIC-LEUKEMIA ANTIGEN
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DOI：
10.1038/283583a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHLOSSMAN, SF
中科院分区：
文献类型：
--
作者：
RITZ, J;PESANDO, JM;SCHLOSSMAN, SF
Greatleman 1和其他人2 - 6先前的研究已经证明，存在与许多急性淋巴细胞白血病（ALL）患者和一些处于急变期的慢性粒细胞白血病（CML）患者的细胞相关的抗原。这种常见ALL抗原（CALLA）的抗血清已在兔子中产生，需要大量吸收，这限制了可以产生的抗血清的滴度和数量，并可能导致不同实验室的特异性不同。由科勒和Milstein 7介绍的体细胞杂交产生特异性抗体的方法已成功地用于产生针对多种正常人细胞表面蛋白的单克隆抗体，包括β 2微球蛋白8、组织相容性抗原9、胸腺细胞和外周T细胞抗原10 - 12和Ia样抗原13。本报告描述了一个共同的ALL抗原（CALLA）特异性的单克隆抗体的产生和表征，以前确定的常规异源抗血清。
Previous studies by Greaves1and others2–6have demonstrated the existence of an antigen associated with cells from many patients with acute lymphoblastic leukaemia (ALL) and some patients with chronic myelocytic leukaemia (CML) in blast crisis. Antisera to this common ALL antigen (CALLA) have been produced in rabbits and require extensive absorption which limits both the titre and quantity of antisera that can be generated and may result in variable specificity in different laboratories. The method for generation of specific antibody by somatic cell hybridisation introduced by Kohler and Milstein7has been successfully used to produce monoclonal antibodies against various normal human cell-surface proteins, includingβ2microglobulin8, histocompatibility antigens9, thymocyte and peripheral T-cell antigens10–12and Ia-like antigens13. The present report describes the generation and characterisation of a monoclonal antibody specific for a common ALL antigen (CALLA) previously identified by conventional heteroantisera.