The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier.
The I.3.2 developmental mutant has a single nucleotide deletion in the gene centromere identifier.
复制标题
DOI：
10.17912/micropub.biology.000653
复制
发表时间：
2022
影响因子：
--
通讯作者：
Kagey, Jacob D
中科院分区：
文献类型：
--
作者：
Evans, Cory J;Bieser, Kayla L;Acevedo-Vasquez, Katherine S;Augustine, Emyli J;Bowen, Skyler;Casarez, Veronica A;Feliciano, Vanessa I;Glazier, Ashley;Guinan, Haley R;Hallman, Randy;Haugan, Elizabeth;Hehr, Lauren A;Hunnicutt, Shawna N;Leifer, Isabella;Mauger, Meaghan;Mauger, Morgan;Melendez, Norma Y;Milshteyn, Larry;Moore, Eric;Nguyen, Sarah A;Phanphouvong, Sierra C;Pinal, David M;Pope, Hailee M;Salinas, Mark-Brandon M;Shellin, Matthew;Small, Ivana;Yeoh, Neelufar C;Yokomizo, Alexandra M K;Kagey, Jacob D
突变I.3.2先前在染色体2 R的条件生长调节剂的FLP/FRT筛选中鉴定。在这里，我们报告的表型表征和遗传图谱的I.3.2的本科生参加的飞行治愈，教学计划，通过课堂研究经验教遗传学的科学。我们发现，创建I.3.2细胞克隆在发展中的眼睛触角成虫盘导致无头成人表型，暗示自主和非自主的影响细胞生长或活力。我们还确定了I.3.2突变作为基因着丝粒标识符（cid）的功能丧失等位基因，该基因编码对染色体分离至关重要的着丝粒特异性组蛋白H3变体。
The mutation I.3.2 was previously identified in a FLP/FRT screen of chromosome 2R for conditional growth regulators. Here we report the phenotypic characterization and genetic mapping of I.3.2 by undergraduate students participating in Fly-CURE, a pedagogical program that teaches the science of genetics through a classroom research experience. We find that creation of I.3.2 cell clones in the developing eye-antennal imaginal disc causes a headless adult phenotype, suggestive of both autonomous and non-autonomous effects on cell growth or viability. We also identify the I.3.2 mutation as a loss-of-function allele of the gene centromere identifier ( cid ), which encodes centromere-specific histone H3 variant critical for chromosomal segregation.