Ginsenosides regulate adventitious root formation in Panax ginseng via a CLE45-WOX11 regulatory module
Ginsenosides regulate adventitious root formation in Panax ginseng via a CLE45-WOX11 regulatory module
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人参皂苷通过 CLE45-WOX11 调节模块调节人参不定根的形成
DOI：
10.1093/jxb/eraa375
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发表时间：
2020-10-22
影响因子：
6.9
通讯作者：
Huang, Luqi
中科院分区：
文献类型：
--
作者：
Liu, Juan;Chen, Tong;Huang, Luqi
不定根分枝对植物的生长和再生至关重要，但这一过程的调控尚不清楚。因此，我们研究了人参皂苷如何调节人参不定根分枝。细胞增殖和不定根分枝的存在下，抑制Rb 1和高浓度的Rb 1 Re，但与低浓度的Re处理时增加。此外，外源性应用的合成十二氨基酸肽，具有CLAVATA 3/胚胎周围区域相关（CLE）的基序对应于PgCLE 45延缓人参和拟南芥的根生长。根Re水平和DDS，CYP 716 A47和CYP 716 A53基因的表达，编码酶参与β-内酰胺酶合成的PgCLE 45的存在下下降。测定PgWOX和PgCLE基因的表达谱以进一步研究CLE-WOX信号通路。PgWOX 11转录物的水平显示出与PgCLE 45转录物相反的模式。使用酵母单杂交试验，EMSA和ChIP试验，我们表明PgWOX 11结合到含有HD基序的PgCLE 45启动子。瞬时表达实验表明，PgWOX 11诱导PgCLE 45在不定根中表达，而PgCLE 45抑制PgWOX 11的表达。这些结果表明，PgCLE 45和PgWOX 11之间存在负反馈调节。综上所述，这些数据表明，黄芪皂苷通过一种新的PgCLE 45-PgWOX 11调节环调节不定根分枝，为不定根分枝的调节提供了一种潜在的机制。
Adventitious root branching is vital to plant growth and regeneration, but the regulation of this process remains un-clear. We therefore investigated how ginsenosides regulate adventitious root branching in Panax ginseng. Cell proliferation and adventitious root branching were decreased in the presence of ginsenoside Rb1 and a high concentration of ginsenoside Re, but increased when treating with a low concentration of Re. Moreover, the exogenous application of a synthetic dodeca-amino acid peptide that has a CLAVATA3/EMBRYO SURROUNDING REGION-related (CLE) motif corresponding to PgCLE45 retarded root growth in both ginseng and Arabidopsis. The root Re levels and the expression of the DDS, CYP716A47, and CYP716A53 genes that encode enzymes involved in ginsenoside synthesis were decreased in the presence of PgCLE45. The expression profiles of PgWOX and PgCLE genes were determined to further investigate the CLE-WOX signaling pathway. The levels of PgWOX11 transcripts showed an inverse pattern to PgCLE45 transcripts. Using yeast one-hybrid assay, EMSA, and ChIP assay, we showed that PgWOX11 bound to the PgCLE45 promoter, which contained the HD motif. Transient expression assay showed that PgWOX11 induced the expression of PgCLE45 in adventitious roots, while PgCLE45 suppressed the expression of PgWOX11. These results suggest that there is a negative feedback regulation between PgCLE45 and PgWOX11. Taken together, these data show that ginsenosides regulate adventitious root branching via a novel PgCLE45-PgWOX11 regulatory loop, providing a potential mechanism for the regulation of adventitious root branching.