Immunohistochemical detection of steroid receptor cofactors in ovarian endometriosis: involvement of down-regulated SRC-1 expression in the limited growth activity of the endometriotic epithelium
Immunohistochemical detection of steroid receptor cofactors in ovarian endometriosis: involvement of down-regulated SRC-1 expression in the limited growth activity of the endometriotic epithelium
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DOI：
10.1007/s00428-010-0884-x
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发表时间：
2010-02
期刊：
影响因子：
3.5
通讯作者：
A. Suzuki;A. Horiuchi;Kenji Oka;T. Miyamoto;H. Kashima;T. Shiozawa
中科院分区：
文献类型：
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作者：
A. Suzuki;A. Horiuchi;Kenji Oka;T. Miyamoto;H. Kashima;T. Shiozawa
为探讨类固醇激素诱导子宫内膜异位症的生长机制，应用免疫组化方法检测了37例子宫内膜异位症上皮中类固醇激素受体辅因子的表达，并与同一患者的在位内膜进行比较。类固醇受体辅激活因子（p300/CBP和SRC-1）和辅抑制因子（NCoR和SMRT）的表达与雌激素受体（ER），孕激素受体（PR）和Ki-67的关系进行了检查。免疫染色结果表示为“阳性指数”（PI，满分; 100）。增生期上皮ER、PR的表达与在位上皮相似，而Ki-67的表达（PI 13.8 ± 2.4，mean ± SD）明显低于在位上皮（32.6 ± 10.6）。SRC-1在正常子宫内膜中的表达在增殖期增加（56.5 ± 16.8），分泌期减少（14.8 ± 6.9）。然而，在子宫内膜异位症中，SRC-1的PI在月经周期中没有表现出明显的周期性变化。SRC-1在增生期上皮中的表达明显低于在位上皮。这些结果表明，减少增生性上皮细胞的增殖活性与SRC-1的表达减少。
To study the steroid hormone-induced growth mechanisms of endometriosis, the immunohistochemical expression of steroid hormone receptor cofactors was investigated in 37 cases of endometriotic epithelia and was compared with that of eutopic endometria of identical patients. The expression of steroid receptor coactivators (p300/CBP and SRC-1) and corepressors (NCoR and SMRT) was examined in relation to the estrogen receptor (ER), the progesterone receptor (PR), and Ki-67. Results of immunostaining were indicated as a “positivity index” (PI, full score; 100). The expression of ER and PR in endometriotic epithelia largely resembled that in eutopic endometria, however, the expression of Ki-67 in the proliferative phase (PI 13.8 ± 2.4, mean ± SD) was significantly lower than that in eutopic endometria (32.6 ± 10.6). The expression of SRC-1 in eutopic endometria was increased in the proliferative phase (56.5 ± 16.8) and decreased in the secretory phase (14.8 ± 6.9). In endometriosis, however, the PI for SRC-1 did not show apparent cyclic changes during the menstrual cycle. Moreover, the expression of SRC-1 in endometriotic epithelia in the proliferative phase was significantly lower than that in eutopic endometria. These findings suggested the reduced proliferative activity in endometriotic epithelia to be related to the reduced expression of SRC-1.