Oxylipin metabolism in soybean seeds containing different sets of lipoxygenase isozymes after homogenization
Oxylipin metabolism in soybean seeds containing different sets of lipoxygenase isozymes after homogenization
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DOI：
10.1271/bbb.60121
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发表时间：
2006-11-01
影响因子：
1.6
通讯作者：
Matsui, Kenji
中科院分区：
文献类型：
--
作者：
Pulvera, Zinna Marie;Kitamura, Keisuke;Matsui, Kenji
在含有不同组脂氧合酶同工酶（L-1、-2和-3）的大豆匀浆中分析了氧脂素代谢;即Suzuyutaka（含有L-1、-2和-3）、Yumeyutaka（仅含有L-1）、Kanto 102（含有L-2）、Kyushu 119（含有L-3）和Ichihime（缺乏所有三种同工酶）。各品种氧化脂肪酸的酯化形式含量均高于游离形式。关东102形成的氧化脂质量最高，Yumeyutaka和Ichihime形成的氧化脂质量最低。对于Kanto 102和Kyushu 119，形成了大量的酮脂肪酸，而对于Yumeyutaka和Ichihime则检测不到。由于Ichihime中缺乏脂氧合酶，预计游离脂肪酸会积累;然而，Yumeyutaka中的游离脂肪酸量显著低于预期。这表明，存在一个途径，形成C6-挥发物通过氢过氧化物的酯化形式。
The oxylipin metabolism was analyzed in soybean homogenates containing different sets of lipoxygenase isozymes (L-1, -2, and -3); namely, Suzuyutaka (containing L-1, -2, and -3), Yumeyutaka (containing only L-I), Kanto102 (containing L-2), Kyushu119 (containing L-3), and Ichihime (lacking all three isozymes). The amount of oxidized fatty acids in the esterified form was higher than that in the free form with every cultivar. Kanto102 formed the highest amount of oxidized lipids, and Yumeyutaka and Ichihime formed the lowest. With Kanto102 and Kyushu119, high amounts of keto fatty acids were formed, while they were undetectable with Yumeyutaka and Ichihime. Due to the lack of lipoxygenases in Ichihime, an accumulation of free fatty acids was expected; however, their amount in Yumeyutaka was significantly lower than was expected. It is suggested that a pathway existed to form C6-volatiles through hydroperoxides in the esterified form.