A novel repressor of floral transition, MEE3, an abiotic stress regulated protein, functions as an activator of FLC by binding to its promoter in Arabidopsis
A novel repressor of floral transition, MEE3, an abiotic stress regulated protein, functions as an activator of FLC by binding to its promoter in Arabidopsis
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MEE3 是一种新型的花转变抑制因子，是一种非生物胁迫调节蛋白，通过与拟南芥中的 FLC 启动子结合，发挥 FLC 激活剂的作用
DOI：
10.1016/j.envexpbot.2014.12.003
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发表时间：
2015-05-01
影响因子：
5.7
通讯作者：
Fan, Liu-Min
中科院分区：
文献类型：
--
作者：
Li, Chaonan;Zhou, Yangyang;Fan, Liu-Min
花转变是有花植物生活史中有性生殖的关键步骤。FLOWERLOCUST（FT）是一种成花启动子和整合子，FLOWERLOCUSC（FLC）是一种成花阻遏子，抑制多种成花启动子，其中包括拟南芥胚胎抑制蛋白3（MEE 3）。MEE 3被认为是MYB相关家族中的I-box-binding-like蛋白的成员，最初被简要报道控制胚胎发育，但没有详细描述。MEE 3是否以及如何参与花的转变仍然完全未知。在这项工作中，我们第一次表明，MEE 3可能作为一个新的抑制剂的花转变作为一个激活PLC通过结合到其启动子在拟南芥。在长日照和短日照条件下，MEE 3的过量表达均导致开花时间延迟。RNA-seq和RT-qPCR分析表明，MEE 3上调PLC和下调FT和其他一些花转换和花分生组织身份基因。遗传分析表明，MEE 3抑制花转变，可能是FLC的上游。酵母单杂交、EMSA和ChIP-qPCR测定的进一步组合证明MEE 3与FLC的启动子结合。(C)2015年由Elsevier B. V.出版
The floral transition is a key step for the sexual reproduction in the life cycle of flowering plants. FLOWERING LOCUST (FT), is known as a floral promoter and integrator while FLOWERING LOCUS C (FLC) is a floral repressor inhibiting several floral promoters including Fr. Arabidopsis MATERNAL EFFECT EMBRYO ARREST 3 (MEE3), predicted to be a member of I-box-binding-like proteins in MYB-related family, was initially briefly reported to control embryo development but without detailed description. Whether and how MEE3 is involved in floral transition remains totally unknown. In this work, we show for the first time that MEE3 may act as a novel repressor of floral transition by functioning as an activator of PLC via binding to its promoter in Arabidopsis. Overexpression of MEE3 caused a delay in flowering time under both long-day and short-day conditions. RNA-seq and RT-qPCR assays revealed that MEE3 upregulated PLC and downregulated FT and some other floral transition and floral meristem identity genes. Genetic analyses showed that MEE3 repressed floral transition and was likely upstream of FLC. A further combination of yeast one-hybrid, EMSA and ChIP-qPCR assays demonstrated that MEE3 is bound to the promoter of FLC. (C) 2015 Published by Elsevier B.V.