Transient expression of laminin α1 chain in regenerating murine liver:: Restricted localization of laminin chains and nidogen-1
Transient expression of laminin α1 chain in regenerating murine liver:: Restricted localization of laminin chains and nidogen-1
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DOI：
10.1016/j.yexcr.2005.01.005
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发表时间：
2005-04-15
影响因子：
3.7
通讯作者：
Mitaka, T
中科院分区：
文献类型：
--
作者：
Kikkawa, Y;Mochizuki, Y;Mitaka, T
根据电子显微镜的定义，上皮细胞和内皮细胞之间的大多数间质都含有基底层 (BL)。然而，在肝脏中，肝细胞和肝窦内皮细胞 (SEC) 之间的肝窦间质（称为迪斯间隙）缺乏 BL。由于层粘连蛋白是全身BL的主要成分，因此层粘连蛋白是否存在于血窦中一直是一个有争议的问题。尽管最近取得了进展，但层粘连蛋白链在哺乳动物肝脏中的分布和表达尚未得到很好的确定。在这里，我们使用一组抗体检查了正常和再生小鼠肝脏中的层粘连蛋白。在α链中，除正弦曲线外，α5在所有BL中广泛观察到，而其他α链在Glisson鞘和中央静脉中表达不同。层粘连蛋白γ1也分布到除sirmsoids之外的所有BL。尽管在所有 BL 和正弦曲线中均观察到 β 2 链，但 β 1 链的表达仅限于 Glisson 鞘。对再生肝脏的详细分析表明，α1 和γ1 链出现在血窦中，并由星状细胞产生。 α1 和 γ1 链的染色在三分之二部分肝切除术 (PHx) 后 6 天达到最大强度。此外，体外研究表明，含α1的层粘连蛋白促进从正常肝脏分离的肝窦内皮细胞（SEC）的扩散，但不促进其他肝细胞的扩散。此外，从再生肝脏中分离出的 SEC 在含有 α1 的 larninin 上延长了伪足，其效果比来自正常肝脏的细胞更明显。层粘连蛋白 α 1 的瞬时表达可能促进 PHx 后血窦的形成。 (c) 2005 Elsevier Inc. 保留所有权利。
Most interstitia between epithelial and endothelial cells contain basal laminae (BLs), as defined by electron microscopy. However, in liver, the sinusoidal interstitium (called space of Disse) between hepatocytes and sinusoidal endothelial cells (SECs) lacks BLs. Because laminins are major components of BLs throughout the body, whether laminins exist in sinusoids has been a controversial issue. Despite recent advances, the distribution and expression of laminin chains have not been well defined in mammalian liver. Here, using a panel of antibodies, we examined laminins in normal and regenerating mouse livers. Of alpha chains, alpha 5 was widely observed in all BLs except for sinusoids, while the other alpha chains were variously expressed in Glisson's sheath and central veins. Laminin gamma 1 was also distributed to all BLs except for sirmsoids. Although the beta 2 chain was observed in all BLs and sinusoids, the expression of beta 1 chain was restricted to Glisson's sheath. Detailed analysis of regenerating liver revealed that alpha 1 and gamma 1 chains appeared in sinusoids and were produced by stellate cells. The staining of alpha 1 and gamma 1 chains reached its maximum intensity at 6 days after two-thirds partial hepatectomy (PHx). Moreover, in vitro studies showed that alpha 1-containing laminin promoted spreading of sinusoidal endothelial cells (SECs) isolated from normal liver, but not other hepatic cells. In addition, SECs isolated from regenerating liver elongated pseudopodia on alpha 1-containing larninin more so than did cells from normal liver. The transient expression of laminin alpha 1 may promote formation of sinusoids after PHx. (c) 2005 Elsevier Inc. All rights reserved.