Multigene Methylation Analysis of Conventional Renal Cell Carcinoma
Multigene Methylation Analysis of Conventional Renal Cell Carcinoma
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DOI：
10.1159/000224878
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发表时间：
2009-01-01
影响因子：
1.6
通讯作者：
Ozkinay, F.
中科院分区：
文献类型：
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作者：
Onay, H.;Pehlivan, S.;Ozkinay, F.
肾细胞癌（RCC）是肾脏最常见的恶性肿瘤。由于肾细胞癌局限于肾包膜时是可以治愈的，因此早期诊断极为重要。启动子高甲基化是人类癌症发展过程中肿瘤抑制基因（TSG）失活的最常见机制。本研究旨在研究 RCC 患者 3 个不同组织样本（正常、癌前、恶性）中 7 个 TSG（RASSF1A、ECAD、TIMP3、APC、MGMT、p16 和 RAR beta 2）的甲基化谱。该研究纳入了 21 名被诊断患有 RCC 的患者。进行甲基化特异性聚合酶链反应来检测 7 TSG 的甲基化模式。在 RCC 患者中观察到基因 RASSF1A (76%)、p16 (80%)、ECAD (42%)、TIMP3 (33%) 和 MGMT (33%) 的高甲基化率。 APC (14%) 和 RAR beta 2 (19%) 基因显示出较低的甲基化率。总之，5 个 TSG（RASSF1A、ECAD、TIMP3、MGMT 和 p16）在 RCC 患者中表现出高甲基化率。包含这些基因的基于甲基化的基因测试可能有助于肾细胞癌的早期检测。版权所有 (C) 2009 S. Karger AG，巴塞尔
Renal cell carcinoma (RCC) is the most common malignancy of the kidney. Since RCC is curable when it is confined to the renal capsule, early diagnosis is extremely important. Promoter hypermethylation is the most common mechanism for the inactivation of the tumor suppressor genes (TSG) in the development of human cancer. This study aimed to investigate the methylation profiles of 7 TSG (RASSF1A, ECAD, TIMP3, APC, MGMT, p16 and RAR beta 2) in 3 different tissue samples (normal, premalign, malign) of patients with RCC. Twenty-one patients diagnosed with RCC were included in the study. Methylation-specific polymerase chain reaction was performed to detect the methylation patterns of the 7 TSG. High methylation rates for the genes RASSF1A (76%), p16 (80%), ECAD (42%), TIMP3 (33%) and MGMT (33%) were observed in the patients with RCC. The APC (14%) and RAR beta 2 (19%) genes showed low methylation rates. In conclusion, 5 TSG (RASSF1A, ECAD, TIMP3, MGMT and p16) showed high methylation rates in RCC patients. A methylation-based gene test including these genes may be useful in the early detection of RCC. Copyright (C) 2009 S. Karger AG, Basel