Delayed flowering time in Arabidopsis and Brassica rapa by the overexpression of FLOWERING LOCUS C (FLC) homologs isolated from Chinese cabbage (Brassica rapa L. ssp pekinensis)
Delayed flowering time in Arabidopsis and Brassica rapa by the overexpression of FLOWERING LOCUS C (FLC) homologs isolated from Chinese cabbage (Brassica rapa L. ssp pekinensis)
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DOI：
10.1007/s00299-006-0243-1
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发表时间：
2007-03-01
影响因子：
6.2
通讯作者：
Lee, Yeon-Hee
中科院分区：
文献类型：
--
作者：
Kim, Soo-Yun;Park, Beom-Seok;Lee, Yeon-Hee
大白菜植物保持在营养生长阶段，直到他们经历了长期暴露在寒冷的温度，称为春化。这种开花抑制是由高水平的FLC表达引起的。为了通过抑制大白菜的成花转变来提高大白菜的产品价值，从大白菜“赤福”中克隆了3个与AtFLC基因同源的成花抑制子基因（BrFLC1、BrFLC2和BrFLC3）。这些基因表现出高度的相似性AtFLC，虽然推定的BrFLC1蛋白含有10个以上的残基比AtFLC。除了BrFLC3在根中不表达外，BrFLC基因普遍表达。BrFLC1和BrFLC2在未春化和春化的大白菜中的表达量均高于BrFLC3。这3个BrFLC基因在早花大白菜中的表达量均较低，表明BrFLC基因的转录水平与开花时间有关。组成型表达的BrFLC基因在拟南芥显着延迟开花，这也被观察到在转基因大白菜过表达BrFLC 3。这些结果表明，BrFLC基因的作用类似于AtFLC。我们的研究结果提供了一种控制开花时间在大白菜和其他作物产生高产量的营养组织的技术。
Chinese cabbage plants remain in the vegetative growth phase until they have experienced prolonged exposure to cold temperature, known as vernalization. This inhibition of flowering is caused by the high levels of FLOWERING LOCUS C (FLC) expression. To increase the product value of Chinese cabbage by inhibiting the floral transition, three genes (BrFLC1, BrFLC2, and BrFLC3) homologous to the AtFLC gene, which encodes a floral repressor, were isolated from the Chinese cabbage 'Chiifu'. These genes showed high similarity to AtFLC, although the putative BrFLC1 protein contained ten more residues than AtFLC. The BrFLC genes were expressed ubiquitously, except that BrFLC3 was not expressed in roots. BrFLC1 and BrFLC2 showed stronger expression than BrFLC3 in unvernalized and vernalized Chinese cabbage. The expression levels of the three BrFLC genes were lower in an early-flowering Chinese cabbage, suggesting that the BrFLC transcript level was associated with flowering time. Constitutive expression of the BrFLC genes in Arabidopsis significantly delayed flowering, which was also observed in transgenic Chinese cabbage overexpressing BrFLC3. These results suggest that the BrFLC genes act similarly to AtFLC. Our results provide a technique for controlling flowering time in Chinese cabbage and other crops to produce high yields of vegetative tissues.