Stretch induced hyperexcitability of mice callosal pathway.
Stretch induced hyperexcitability of mice callosal pathway.
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DOI：
10.3389/fncel.2015.00292
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发表时间：
2015
影响因子：
5.3
通讯作者：
Saif T
中科院分区：
文献类型：
--
作者：
Fan A;Stebbings KA;Llano DA;Saif T
记忆和学习被认为是突触强度变化的结果。以往对脑切片中突触生理学的研究传统上集中在生物化学过程上。在这里，我们用小鼠脑切片的实验证明，中枢神经系统的可塑性也对机械拉伸敏感。这一点很重要，因为许多临床情况都涉及大脑机械张力的变化，以及机械张力在大脑发育中的正常作用。开发了一个新的平台来研究神经对机械拉伸的反应。黄素蛋白自发荧光（FA）成像用于测量神经活动。我们观察到，突触兴奋性大幅增加后，一个小的（2.5%）拉伸举行了10分钟，并释放。增加是累积的，即，多次拉伸循环进一步增加了兴奋性。我们还开发了分析工具来量化空间分布和响应强度。结果表明，在经历拉伸-非拉伸循环的切片中，空间扩展不太稳定。FA幅度和激活率下降，兴奋性增加，在拉伸的情况下，但不是在电增强的情况下。这些结果共同表明，在生理范围内的一个小的拉伸可以调节神经活动显着，这表明，机械事件可以作为一种新的工具，用于神经可塑性的调制。
Memory and learning are thought to result from changes in synaptic strength. Previous studies on synaptic physiology in brain slices have traditionally been focused on biochemical processes. Here, we demonstrate with experiments on mouse brain slices that central nervous system plasticity is also sensitive to mechanical stretch. This is important, given the host of clinical conditions involving changes in mechanical tension on the brain, and the normal role that mechanical tension plays in brain development. A novel platform is developed to investigate neural responses to mechanical stretching. Flavoprotein autofluoresence (FA) imaging was employed for measuring neural activity. We observed that synaptic excitability substantially increases after a small (2.5%) stretch was held for 10 min and released. The increase is accumulative, i.e., multiple stretch cycles further increase the excitability. We also developed analytical tools to quantify the spatial spread and response strength. Results show that the spatial spread is less stable in slices undergoing the stretch-unstretch cycle. FA amplitude and activation rate decrease as excitability increases in stretch cases but not in electrically enhanced cases. These results collectively demonstrate that a small stretch in physiological range can modulate neural activities significantly, suggesting that mechanical events can be employed as a novel tool for the modulation of neural plasticity.